Book chapter
Isolation and growth of rhizobia
Working with rhizobia, pp.39-60
Australian Centre for International Agricultural Research
2016
Abstract
This chapter describes basic techniques for the isolation and growth of rhizobia, some of which have been used for more than a century. While these techniques re¬tain their importance, the success of current and future rhizobiology studies and enterprises will depend on the training, skills and techniques described in this chapter. A note of caution: nodules (particularly those collected from the field) are not always occupied by a single rhizobial isolate nor even by a single micro-or¬ganism. Nodules of pea and lupin, for example, have been described as containing both the nitrogen-fixing symbiont and associative organisms such as Micromono¬spora (Trujillo et al. 2010). Hence, we must be prepared for a range of organisms to appear on growth plates during isolation procedures. Recognition of rhizobia when growing on a solid medium is an essential skill in rhizobiology.
Details
- Title
- Isolation and growth of rhizobia
- Authors/Creators
- M. Hungria (Author/Creator)G.W. O'Hara (Author/Creator)J.E. Zilli (Author/Creator)R.S. Araujo (Author/Creator)R. Deaker (Author/Creator)J.G. Howieson (Author/Creator)
- Contributors
- J.G. Howieson (Editor)M.J. Dilworth (Editor)
- Publication Details
- Working with rhizobia, pp.39-60
- Publisher
- Australian Centre for International Agricultural Research; Canberra
- Identifiers
- 991005540254907891
- Copyright
- © Australian Centre for International Agricultural Research (ACIAR) 2016
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Book chapter
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