Book chapter
Masculinization of Zebrafish through partial depletion of primordial germ by injecting diluted oligonucleotides into Embryos
Germline Development in the Zebrafish, Vol.2218, pp.49-60
Humana Press
2021
Abstract
The regulation of reproduction in zebrafish, the prime model of fish research, is not fully understood. An efficient tool to gain a better understanding of this complicated process is utilization of severely sex-biased families or groups. Here, we describe a method for partial depletion of primordial germ cells (PGCs) that leads to eventual masculinization of zebrafish. The technique is based on injecting early embryos with diluted morpholino oligonucleotides that temporarily interfere with the production of Dead end (Dnd), an RNA-binding protein essential for PGC survival. In addition, we also propose the use of eviscerated trunk, as a suitable alternative for examining gonadal expression in juvenile zebrafish.
Details
- Title
- Masculinization of Zebrafish through partial depletion of primordial germ by injecting diluted oligonucleotides into Embryos
- Authors/Creators
- L. Orbán (Author/Creator) - Murdoch UniversityJ.M. Saju (Author/Creator) - Temasek Life Sciences LaboratoryK-W Tzung (Author/Creator) - A*STAR Graduate AcademyW.C. Liew (Author/Creator) - Temasek Life Sciences Laboratory
- Contributors
- R. Dosch (Editor)
- Publication Details
- Germline Development in the Zebrafish, Vol.2218, pp.49-60
- Publisher
- Humana Press
- Identifiers
- 991005540938307891
- Murdoch Affiliation
- Centre for Comparative Genomics
- Language
- English
- Resource Type
- Book chapter
- Additional Information
- Part of the Methods in Molecular Biology book series (MIMB, volume 2218)
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