Book chapter
Mass spectrometry imaging (MSI) for plant metabolomics
Plant Metabolomics, Vol.1778, pp.241-252
Humana Press
2018
Abstract
Mass spectrometry imaging (MSI) is a developing technique to measure the spatiotemporal distribution of many biomolecules in tissues. Over the preceding decade MSI has been adopted by plant biologists and applied in a broad range of areas including: primary metabolism, natural products, plant defense, plant responses to abiotic and biotic stress, plant lipids, and the developing field of spatial metabolomics. This methods chapter covers preparation of plant tissues for matrix-assisted laser desorption ionization (MALDI)-MSI, including sample embedding and freezing, sectioning, mounting, and matrix deposition using both sublimation and spray deposition prior to MSI analysis.
Details
- Title
- Mass spectrometry imaging (MSI) for plant metabolomics
- Authors/Creators
- B.A. Boughton (Author/Creator)D. Thinagaran (Author/Creator)
- Contributors
- C. António (Editor)
- Publication Details
- Plant Metabolomics, Vol.1778, pp.241-252
- Publisher
- Humana Press; New York, NY
- Identifiers
- 991005544748307891
- Copyright
- © Springer Science+Business Media, LLC, part of Springer Nature 2018
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Book chapter
- Additional Information
- Part of the Methods in Molecular Biology book series (MIMB, Volume 1778)
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