Book chapter
Optimizing Splice-Switching Oligomer Sequences Using 2′-O-Methyl Phosphorothioate Chemistry
Exon Skipping: Methods and Protocols, Vol.867, pp.169-188
Humana Press
2012
Abstract
We have taken an empirical approach in designing splice-switching oligomers to induce targeted dystrophin exon skipping. The nucleotide sequence of the exon under examination is first analyzed for potential exon recognition motifs and then a set of oligomers complementary to the acceptor and donor splice sites, as well as intra-exonic regions predicted to contain exon splice enhancers, are designed and synthesized as 2′-O-methyl-modified bases on a phosphorothioate backbone (2OMeAOs). The 2OMeAOs can be readily transfected into cultured normal myogenic cells as cationic lipoplexes, and are incubated for 24 h before total RNA extraction and subsequent analysis by semi-quantitative RT-PCR. The amplification conditions used for each dystrophin transcript region under investigation minimize preferential production of shorter amplicons and do not exaggerate the level of induced RT-PCR products, compared to the endogenous dystrophin transcript product. It is imperative that the test oligomers are transfected over a range of concentrations and that the target exon is excised in a reproducible and dose-dependent manner.
Once it has been demonstrated that an oligomer can induce some degree of exon skipping, that target region of the pre-mRNA is assumed to be involved in splicing of the exon. A series of overlapping oligomers are prepared and evaluated by transfection into normal myogenic cells at lower concentrations to identify the more effective compounds. Clinical application requires antisense compounds that efficiently modulate splicing at low dosages, delivering the greatest benefits in terms of efficacy, safety, and cost.
Details
- Title
- Optimizing Splice-Switching Oligomer Sequences Using 2′-O-Methyl Phosphorothioate Chemistry
- Authors/Creators
- C. Adkin (Author/Creator) - The University of Western AustraliaS. Fletcher (Author/Creator) - The University of Western AustraliaS.D. Wilton (Author/Creator) - The University of Western Australia
- Contributors
- A. Aartsma-Rus (Editor)
- Publication Details
- Exon Skipping: Methods and Protocols, Vol.867, pp.169-188
- Publisher
- Humana Press
- Identifiers
- 991005540081807891
- Copyright
- 2012 Springer Science + Business Media
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Book chapter
- Note
- Series Title: Methods in Molecular Biology, ISSN: 1064-3745; Vol. 867
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