Book chapter
PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene
PCR for Clinical Microbiology: An Australian and International Perspective, pp.407-414
Springer
2010
Abstract
Methicillin resistance, in particular hetero-methicillin resistance, in S aureus and the coagulase negative staphylococci (CNS) can be difficult to detect by phenotypic methods. Subsequently detection of the mecA gene for determining methicillin resistance in staphylococci is generally considered to be the Gold Standard. In Australian diagnostic microbiology laboratories, molecular assays used for the detection of the mecA gene have generally been designed in-house. These assays have either been single primer PCR assays targeting the mecA gene or a duplex primer PCR assay with primers targeting the mecA gene and a gene specific for S. aureus such as the nuc (nuclease) or fem gene. In some laboratories a Panton Valentine-leukocidin (PVL) gene primer has been incorporated with the duplex PCR assay allowing the detection of PVL virulence genes in S aureus.
Details
- Title
- PCR assays in detecting methicillin resistance in staphylococci: Coagulase negative staphylococci (CNS), S. aureus, and MRSA with the PVL gene
- Authors/Creators
- J. Merlino (Author/Creator) - Concord HospitalI.D. Kay (Author/Creator) - Royal Perth HospitalG. Coombs (Author/Creator) - Royal Perth HospitalS. Palladino (Author/Creator) - Royal Perth Hospital
- Contributors
- M. Schuller (Editor)T.P. Sloots (Editor)G.S. James (Editor)C.L. Halliday (Editor)I.W.J. Carter (Editor)
- Publication Details
- PCR for Clinical Microbiology: An Australian and International Perspective, pp.407-414
- Publisher
- Springer; Dordrecht
- Identifiers
- 991005541088107891
- Copyright
- © 2010 Springer Science+Business Media B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Book chapter
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