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Preservation of rhizobia
Book chapter   Open access

Preservation of rhizobia

M. Hungria, J. Ardley, G.W. O'Hara and J.G. Howieson
Working with rhizobia, pp.61-71
Australian Centre for International Agricultural Research
2016
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Abstract

Experimentation with strains of rhizobia can last for many decades; hence there must be a reliable and efficient means of storing the bacteria. While many re¬positories of rhizobia have been developed since the symbiosis was scientifically understood, few remain available for exploitation. This is because strains were commonly stored on agar and remained the responsibility of an enthusiast, who may not necessarily have been replaced by his institution upon retirement. Agar slope-borne cultures have a relatively finite life. For this reason we recommend long-term preservation of valuable cultures lyophilized in glass tubes which will ensure survival over long periods of inattention.

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