Book chapter
Towards developing transgenic resistance to nematodes in wheat
Cereal cyst nematodes: Status, research and outlook. Proceedings of the first Workshop of the International Cereal Cyst Nematode Initiative, 21-23 October 2009, Antalya, Turkey, pp.191-194
CIMMYT
2009
Abstract
Research is in progress to use biotechnological tools to aid development of new forms of diagnostics and resistance to nematodes in wheat. For diagnostics, protein profiling by MALDI-TOF mass spectroscopy has been used to identify a range of nematode species, including seed gall, root-lesion and stem nematodes. Nematode species, and in some cases, races, can be identified and differentiated on the basis of characteristic protein biomarkers. A reproducible assay system for compounds that affect root-lesion nematode replication, based on carrot mini-discs, has been developed. Genomic studies have also been initiated to generate eDNA libraries and genomic sequences of root lesion and cereal cyst-nematodes. For new forms of genetic resistance to nematode pests of cereals, molecular approaches are in progress to identify new gene targets, generation of potential resistance-conferring gene constructs and their introduction into wheat by particle bombardment followed by regeneration and challenge to identify nematode resistant wheat plants.
Details
- Title
- Towards developing transgenic resistance to nematodes in wheat
- Authors/Creators
- M.G.K. Jones (Author/Creator)J. Rana (Author/Creator)J. Fosu-Nyarko (Author/Creator)J. Zhang (Author/Creator)M.R. Perera (Author/Creator)D. Chamberlain (Author/Creator)
- Contributors
- I.T. Riley (Editor)J.M. Nicol (Editor)A.A. Dababat (Editor)
- Publication Details
- Cereal cyst nematodes: Status, research and outlook. Proceedings of the first Workshop of the International Cereal Cyst Nematode Initiative, 21-23 October 2009, Antalya, Turkey, pp.191-194
- Publisher
- CIMMYT; Ankara, Turkey
- Identifiers
- 991005540773707891
- Copyright
- © International Maize and Wheat Improvement Centre (CIMMYT) 2009
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Book chapter
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