Book chapter
cDNA generation on paramagnetic beads
Generation of cDNA Libraries: Methods in Molecular Biology, Vol.221, pp.25-31
Humana Press
2003
Abstract
Synthesis of complementary DNA (cDNA) by reverse transcription (RT) is a key step in investigating specific gene expression of a single transcript by RT-PCR (polymerase chain reaction) or to study the more complex profiles of gene expression in a biological sample using cDNA library or other techniques. Solid-phase generation of cDNA (1), based on immobilization of the synthesized cDNA on paramagnetic beads and the magnetic-bead separation technology, is a very useful method for studying gene expression particularly when handling limited amounts of starting materials (see Note 1). Using this method, analysis of gene expression can be carried out at a single-cell level (2–4). This is not possible using conventional techniques of mRNA isolation and cDNA cloning.
Details
- Title
- cDNA generation on paramagnetic beads
- Authors/Creators
- Z. Wang (Author/Creator) - Murdoch UniversityM.G.K. Jones (Author/Creator)
- Publication Details
- Generation of cDNA Libraries: Methods in Molecular Biology, Vol.221, pp.25-31
- Publisher
- Humana Press; Totowa, NJ
- Identifiers
- 991005541104807891
- Copyright
- © Humana Press Inc.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Book chapter
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