Conference paper
FUZZY LOGIC-BASED MOBILE PHASE OPTIMIZATION AND DIFFERENTIAL ANALYSIS OF LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY DETECTION OF COENZYME Q10 AND 25-HYDROXYVITAMIN D
ICMLC & ICWAPR 2025 (Bali, Indonesia, 12/07/2025–15/07/2025)
07/2025
Abstract
Liquid chromatography-tandem mass spectrometry (LC-MS/MS) serves as a key tool for the test of lipophilic substances in laboratory medicine and is widely employed in the analysis of coenzyme Q10 (CoQ10) and 25-hydroxyvitamin D (25OHD). In this paper, fuzzy concept was applied to improve the LC-MS/MS methods used for CoQ10 and 25OHD detection. The focus was placed on selecting the optimal mobile phase for CoQ10 analysis and examining the differences between LC-MS/MS and chemiluminescence immunoassay (CLIA) methods for 25(OH)D measurement. Through screening various organic phase combinations and employing fuzzy inference, the optimal mobile phase ratio for CoQ10 test is determined to be methanol and isopropanol at a ratio of 8:2. Additionally, fuzzy logic was employed to analyze the variations in 25OHD concentrations across different sexes and age groups. The results showed that women aged 30–40 exhibited greater differences in 25(OH)D levels compared to other groups. This study shows that the use of fuzzy concepts can enhance the adaptability and accuracy of LC-MS/MS detection, offering a novel approach to the analysis of lipophilic substances.
Details
- Title
- FUZZY LOGIC-BASED MOBILE PHASE OPTIMIZATION AND DIFFERENTIAL ANALYSIS OF LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY DETECTION OF COENZYME Q10 AND 25-HYDROXYVITAMIN D
- Authors/Creators
- Hongkun Liu (Author) - Department of Clinical Laboratory, Sichuan Taikang HospitalJianli Cui (Author) - Department of Clinical Laboratory, Sichuan Taikang HospitalKok Wai Wong (Author) - Murdoch University, Centre for Water, Energy and WasteSirui Li (Author) - Murdoch University, School of Information TechnologyXi Huang (Author) - Department of Clinical Laboratory, Sichuan Taikang HospitalYujie Li - Department of Clinical Laboratory, Sichuan Taikang Hospital
- Conference
- ICMLC & ICWAPR 2025 (Bali, Indonesia, 12/07/2025–15/07/2025)
- Identifiers
- 991005824450107891
- Murdoch Affiliation
- School of Information Technology; Centre for Water, Energy and Waste
- Language
- English
- Resource Type
- Conference paper
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