Conference presentation
Aberrant expression of TRAIL in placenta and maternal serum in early pregancy complications
15th International & 14th European Congress of Endocrinology (Florence, Italy, 05/05/2012–09/05/2012)
2012
Abstract
Recurrent miscarriage (RM) is defined as ≥3 consecutive pregnancy losses and occurs in 1–3% of fertile couples. No specific early biomarkers with high predictive value of threatening miscarriage have been identified. Using GeneChips (Affymetrix), we profiled whole-genome differential gene expression in RM placental tissue compared to uncomplicated pregnancies matched for gestational age. In cases of RM, significantly increased placental mRNA expression of TNF-related apoptosis-inducing ligand (TRAIL; P<0.005) and S100A8 (P<0.001) encoding for inflammatory marker calprotectin (S100A8/A9) was confirmed by Taqman RT-qPCR. Using ELISA, the concentrations of soluble TRAIL and calprotectin in maternal serum in normal first trimester (n=35) and failed pregnancies (early, n=18 and late miscarriage, n=4; tubal pregnancy, n=11) were determined. When compared to normal first trimester pregnancy, significantly higher maternal serum concentration of sTRAIL was detected at the RM event (P<0.001), in women with tubal pregnancy (P<0.05), and in pregnant women, who developed an unpredicted miscarriage 2–50 days after prospective serum sampling (P<0.05). Maternal serum levels of calprotectin were neither diagnostic nor prognostic to early pregnancy failures (P>0.05). sTRAIL may have a potential as a predictive biomarker in maternal serum for early pregnancy complications.
Details
- Title
- Aberrant expression of TRAIL in placenta and maternal serum in early pregancy complications
- Authors/Creators
- K. Rull (Author/Creator)K. Tomberg (Author/Creator)S. Kõks (Author/Creator)J. Männik (Author/Creator)M. Möls (Author/Creator)M. Sirotkina (Author/Creator)S. Värv (Author/Creator)M. Laan (Author/Creator)
- Conference
- 15th International & 14th European Congress of Endocrinology (Florence, Italy, 05/05/2012–09/05/2012)
- Identifiers
- 991005546367807891
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Conference presentation
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