Conference presentation
Comparison of baiting and molecular methods for the detection of Phytophthora cinnamomi form soil samples from south Western Australia
15th Australasian Plant Pathology Society Conference (Geelong, Vic., 26/09/2005–29/09/2005)
2005
Abstract
Phytophthora cinnamomi has been responsible for widespread destruction of native forests and heath lands throughout southern Australia. Baiting has been the primary method for detecting p, cinnamomi from soil samples (I). However, it is limited by the common occurrence of false negatives and the inability to detect dormant spores within the soil (2). The introduction of molecular based detection methods offer improved sensitivity and specificity for P. cinnamomi detection (3). PCR amplification is central to various methods of molecular detection including PCR-RFLP and microarray analysis. Several previous studies have compared the theoretical sensitivity of PCR and baiting of zoospore and chlamydospore inoculations without assessing naturally infested soils (4). This study compares baiting to primary and nested PCR detection of P. cinnamomi from naturally infested soils.
Details
- Title
- Comparison of baiting and molecular methods for the detection of Phytophthora cinnamomi form soil samples from south Western Australia
- Authors/Creators
- N.M. Anderson (Author/Creator)G.E.St.J. Hardy (Author/Creator)P.A. O'Brien (Author/Creator)
- Conference
- 15th Australasian Plant Pathology Society Conference (Geelong, Vic., 26/09/2005–29/09/2005)
- Identifiers
- 991005544326607891
- Murdoch Affiliation
- Centre for Phytophthora Science and Management
- Language
- English
- Resource Type
- Conference presentation
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