Conference presentation
HLA-B*5701 Taqman assay for abacavir sensitivity: Application to PREDICT-1 trial
15th International Histocompatibility and Immunogenetics Workshop (IHW) (Rio de Janeiro, Brazil, 13/09/2008–26/09/2008)
2008
Abstract
Aim: Around 5% of HIV infected patients treated with the HIV drug abacavir experience an allergic hypersensitive response within 6 weeks. Genetic association studies have shown that the HLA-B*5701 allele is highly associated with abacavir hypersensitivity (Mallal et al 2002). and studies of in vitro T cell responses to abacavir pulsed APC indicate that the B*5701 allele is a causal determinant of allergic hypersensitivity (Chessman et al 2007). Our aim was to develop a real-time PCR method of detecting the B*5701 allele on the same automated platform (Cobas AmpliPrep/Cobas TaqMan;CAP/CTM) currently used for determining HIV viral load.
Methods: The B*5701 CTM is a single tube, 2 channel research assay using 2 pairs of primers and Hex- and Fam-labeled Taqman probes. A Hex labeled control probe binds to the exon 2 of all HLA-B alleles. The exon 3 primers and the Fam labeled probe are B*5701 specific.
Results: This assay was used to determine the B*5701 status of 1956 DNA samples from the PREDICT-1 study (Mallal et al 2008). This study demonstrated the clinical utility of B*5701 screening prior to abacavir treatment. HLA-B*5701 status was independently determined by using SSOP followed by SBT for HLA-B57 positive samples (LabCorp) as well as SBT single step full allelic typing (Perth). The Roche CTM HLA-B*5701 results are fully concordant with the sequencing results. Currently, this research assay can be run on whole blood in the same integrated CAP/CTM platform used for measuring HIV viral load.
Conclusions: The HLA-B*5701 CAP/CTM assay is a valuable research screening test for determining HLA-B*5701 status in abacavir-naï ve HIV patients.
Details
- Title
- HLA-B*5701 Taqman assay for abacavir sensitivity: Application to PREDICT-1 trial
- Authors/Creators
- T. Bugawan (Author/Creator)W. Isoda (Author/Creator)C. Mano (Author/Creator)A. Blair (Author/Creator)S. Mallal (Author/Creator)D. Thorborn (Author/Creator)H. Erlich (Author/Creator)
- Conference
- 15th International Histocompatibility and Immunogenetics Workshop (IHW) (Rio de Janeiro, Brazil, 13/09/2008–26/09/2008)
- Identifiers
- 991005544790407891
- Murdoch Affiliation
- Centre for Clinical Immunology and Biomedical Statistics
- Language
- English
- Resource Type
- Conference presentation
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