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Disease phenotyping and genotyping analysis data in natural and RIL narrow-leafed lupin populations
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Disease phenotyping and genotyping analysis data in natural and RIL narrow-leafed lupin populations

Gaofeng Zhou and Chengdao Li
Murdoch University
Third Quarter 2030
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MS107_Milestone_MurdochUni_16 May 2025646.76 kB
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Abstract

For anthracnose, the natural population consisting of over 300 accessions were evaluated in South Perth WA 2024. For Unicrop/Tanjil RIL8 population, the pod Phomopsis disease was evaluated in South Perth WA 2024. QTL was conducted for pod Phomopsis. The genotypic data of the natural population was generated from 10X re-sequencing data. The vcf file containing DNA variants was used to conduct the GWAS analysis for anthracnose disease. For each sample, the DNA was extracted from fresh leave of single plant. CTAB method was used for DNA extraction. Re-sequencing depth was around 10X. BWA and samtools, bcftools were used to call the DNA variants.

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