Doctoral Thesis
Characterising genetic recombination associated with the integration of two novel HMW-GS (1Slx2.3+1Sly16) from Aegilops longissima to Australian wheat cultivars
Doctor of Philosophy (PhD), Murdoch University
2022
Abstract
Wheat (Triticum aestivum L., 2n = 6x = 42, AABBDD) is a staple food crop for about 40% of the world population. For improving bread making quality, two novel high molecular weight glutenin subunits, 1S1x2.3 and 1S1y16 from wheat relative Aegilops longissima was integrated into six Australian wheat cultivars that resulted in a better dough and baking quality. SE-HPLC and Near Infrared based investigations demonstrated that grain protein and gluten content were increased in the BC4F6 recombinant lines RLs except Livingston background. Yield and the other agronomic performances of the RLs were evaluated against the recurrent parental cultivars in two field and one glasshouse trials over two years. Considering all the environments, two RLs of the Yitpi background produced higher yields (43.45 and 77.63%) and protein contents (19.53 and 15.71%) compared to Yitpi. While in most cases, the target traits were improved in the RLs, some non-target traits including plant height, flowering time, grain yield and yield components such as grain number per spike, spikelet number per spike and thousand kernel weight exhibited considerable variations compared to their recurrent parents. This finding demonstrated that some of the genetic recombination responsible for those non-target trait variation were not removed by backcrossing, indicating the linkage drag associated with the novel HMW-GSs. High-density 90K SNP array was carried out to identify the genetic recombination across the RLs to characterise the genetic basis of the linkage drag. Candidate genes responsible for the particular trait variation was identified through bioinformatics approaches. As results, the gene TraesCS1B01G232800 on chromosome 1B is potentially contributing to grain number per spike and TraesCS5B01G019600 on chromosome 5B plays a potential role in grain yield.
Details
- Title
- Characterising genetic recombination associated with the integration of two novel HMW-GS (1Slx2.3+1Sly16) from Aegilops longissima to Australian wheat cultivars
- Authors/Creators
- RESAD R Mallik
- Contributors
- Wujun Ma (Supervisor) - Murdoch University, Centre for Crop and Food InnovationShahid Islam (Supervisor) - Murdoch University, Centre for Crop and Food InnovationJingjuan Zhang (Supervisor) - Murdoch University, Centre for Crop and Food Innovation
- Awarding Institution
- Murdoch University; Doctor of Philosophy (PhD)
- Identifiers
- 991005548669707891
- Murdoch Affiliation
- Australia-China Joint Centre for Wheat Improvement; College of Science, Health, Engineering and Education; School of Agricultural Sciences
- Resource Type
- Doctoral Thesis
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