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Comparative genomics analysis and development of bioinformatics tools for two newly sequenced spirochaete species
Doctoral Thesis   Open access

Comparative genomics analysis and development of bioinformatics tools for two newly sequenced spirochaete species

Yair Motro
Doctor of Philosophy (PhD), Murdoch University
2008
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Abstract

The bacterial family Spirochaetales contains a number of potent pathogens responsible for serious and well-known diseases, such as tick Lyme disease (Borrelia burgdoferri), leptospirosis (Leptospira interrogens), and sypillis (Treponema pallidum). Though the mentioned species have been extensively investigated, there still remain spirochaete genera, and the spirochaete family as a whole, that have been minimally characterised. The Brachyspira genera includes species primarily responsible for gastro-intestinal diseases. Some biological characteristics of the two species B. hyodysenteriae and B. pilosicoli are known. For example B. hyodysenteriae causes disease in swine, while B. pilosicoli causes disease in a wide range of animals and humans. As there are no whole genome sequences available for any Brachyspira species, their underlying molecular mechanisms, evolution and function are not understood. This work is part of a large project which aims to sequence the two whole genome sequences for vaccine design and development. This thesis represents the first report of an in-depth comparative genome analysis (CGA) of the novel whole genome sequences of both Brachyspira species, providing greater understanding into their genomic functional relationships, evolution and diversity, while also identifying elements for potential vaccine and drug design and development.

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