Doctoral Thesis
Metabolic Study to Understand Annual Ryegrass Toxicity (ARGT): A Complex Association of Nematode, Bacteria and Ryegrass
Doctor of Philosophy (PhD), Murdoch University
2023
Abstract
Ryegrass is an important forage grass having excellent nutrition, high palatability and more productivity. Due to its nutritional quality and high digestibility, it is used as pasture, hay and silage in livestock feed. However, one of the most constrain in annual ryegrass production is annual ryegrass toxicity (ARGT) which is an often-fatal poisoning to livestock that consume annual ryegrass infected by the bacterium, Rathayibacter toxicus. Since 1968, the deaths of millions of sheep and cattle took place in Australian history and still more than 12 million livestock are at risk in the country. Therefore, it is important to understand metabolic relationship between nematode (Anguina funesta), bacteria and ryegrass and associate with toxin production.
In this project, three phases of investigation were undertaken, the first phase was the development of a technique for detecting the infected ryegrass material and this study demonstrated that DI-SPME is a valid technique to study differentially expressed metabolites in infected and healthy ryegrass seed and may help provide better understanding of the biochemical interactions between plant and pathogen to aid in management of ARGT. The second phase study focused on profiling of volatile organic compounds associated with metabolites form the bacteria and its infested ryegrass seeds, which were identified as potential biomarker to explore chemical communication between bacteria and nematode. The third phase study focused exploration of untargeted metabolites and identified chemicals associated to ARGT resistant and susceptible cultivars of ryegrass. In conclusion, there is significant different between resistant cultivars during chemical profiling of inflorescence, leaves and stem.
The study significantly achieved designed research aims to improve the development programs for ryegrass and manage the disease effectively by identifying potential chemicals that can be used to enhance resistance in plant. The findings of this study have implications for the agricultural industry and provide a valuable contribution to develop sustainable disease management practices.
Details
- Title
- Metabolic Study to Understand Annual Ryegrass Toxicity (ARGT): A Complex Association of Nematode, Bacteria and Ryegrass
- Authors/Creators
- Pushpendra Koli
- Contributors
- Simon McKirdy (Supervisor) - Murdoch University, Centre for Biosecurity and One HealthYonglin Ren (Supervisor) - Murdoch University, Centre for Biosecurity and One HealthDavid Kessell (Supervisor) - Department of Primary Industries and Regional DevelopmentManjree Agarwal (Supervisor) - Murdoch University
- Awarding Institution
- Murdoch University; Doctor of Philosophy (PhD)
- Identifiers
- 991005628868107891
- Murdoch Affiliation
- College of Environmental and Life Sciences; School of Agricultural Sciences
- Resource Type
- Doctoral Thesis
UN Sustainable Development Goals (SDGs)
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