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A Comprehensive Review of Various Substrates Commonly Encountered at Crime Scenes and their Fluorescence Characteristics using the Polilight Flare® Plus 2
Thesis   Open access

A Comprehensive Review of Various Substrates Commonly Encountered at Crime Scenes and their Fluorescence Characteristics using the Polilight Flare® Plus 2

Emma Howard
Masters by Coursework, Murdoch University
2018
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Whole Thesis Open Access

Abstract

Biological fluids are often present within a crime scene and can be a valuable source of DNA evidence; therefore the detection and identification of these fluids is critical. Alternative light sources are a presumptive testing tool that can be used to detect and enhance potential biological and non-biological staining, through the exploitation of the ultraviolet, visible light and infrared portion of the electromagnetic spectrum. Most biological fluids will emit fluorescence when excited by light from an alternative light source; however previous research has demonstrated that a range of non-biological products will also display fluorescence when viewed under alternative light sources, potentially causing confusion within a crime scene. Further investigation is required to develop a better understanding of the fluorescence displayed by biological and nonbiological products under a wide range of wavelengths and interference filters. This review will assess the current literature regarding the use of alternative light sources for the detection and visualisation of biological fluids, focusing on past studies that have recognised potential complications, such as the fluorescence of non-biological materials and the influence of background substrates on the detection of stains.

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