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Co-extraction methods of DNA and total RNA for body fluid identification: A review
Thesis   Open access

Co-extraction methods of DNA and total RNA for body fluid identification: A review

Anh Tran
Masters by Coursework, Murdoch University
2020
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Abstract

Body fluid identification is a crucial step in forensic investigation, and investigators have used various techniques. With the discovery of cell-specific mRNA and miRNA markers as well as their stability in certain conditions, co-analysis of DNA and RNA from the same sample is possible and can bring multiple benefits. The workflow can be streamlined and the frequent lack of forensic sample will no longer be an obstacle. The need for a robust co-extraction method of DNA and total RNA is clear and there are several kits on the market to serve this need. This work aimed to establish an overview of nucleic acid extraction and dual extraction processes for body fluid identification as well as the use of commercial co-extraction kits.

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