Thesis
Development of a Large Volume DNA Extraction Method
Masters by Coursework, Murdoch University
2025
Abstract
Efficient DNA extraction from large-volume forensic samples remains a critical challenge, undermining the reliability of downstream analyses essential for successful forensic DNA profiling. Conventional extraction methods, while effective for small-volume samples, often demonstrate reduced efficiency, suboptimal DNA recovery, and heightened contamination risks when applied to larger sample volumes. This literature review provides a comprehensive examination of current and emerging DNA extraction techniques, with a particular emphasis on the unique challenges posed by large-volume forensic samples. Through a critical evaluation of the advantages and limitations of these methods, this review identifies key gaps in the literature, including the dependency of DNA recovery success on sample type, substrate, and extraction parameters. This review consolidates existing knowledge and advocates for the development of innovative, scalable extraction protocols that maximise DNA recovery while preserving sample integrity. By addressing these challenges, future research can advance the field towards more efficient, reliable, and scalable methodologies for large-volume forensic DNA extraction methods, ultimately enhancing the accuracy and reliability of forensic DNA analysis for a range of larger substrates.
Details
- Title
- Development of a Large Volume DNA Extraction Method
- Authors/Creators
- Natasha Banks
- Contributors
- Marie Rye (Supervisor)Brendan Chapman (Supervisor) - Murdoch University, School of Medical, Molecular and Forensic SciencesColby Hymus (Supervisor)
- Awarding Institution
- Murdoch University; Masters by Coursework
- Identifiers
- 991005831950307891
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Resource Type
- Thesis
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