Thesis
Influence of Time on DNA Extraction Yield and Test Outcomes from HemaTrace® and RSID-Semen™ Buffers
Masters by Coursework, Murdoch University
2025
Abstract
Identifying body fluids at crime scenes plays a crucial role in forensic sciences and in directing criminal investigations. Fluids such as blood and semen are frequently recovered biological fluids at crime scenes, and identifying these fluids provides crucial preliminary information that guides subsequent forensic analyses, including DNA profiling. Immunochromatographic tests (ICT) such as ABAcardR HemaTraceR and RSID-Semen™ are kits utilised for the forensic identification of human haemoglobin and human semenogelin to determine the presence of biological fluids. When the primary sample for downstream DNA analysis is inadequate, DNA could be extracted from the buffers of HemaTraceR and RSID-Semen™ kits to create an STR profile. This review aimed to investigate and outline DNA extraction methodologies within forensic fields, the structure and function of immunochromatographic tests with a specific focus on HemaTraceR and RSIDSemen ™ kits and buffers, DNA extraction from ICTs both within biomedical and forensic fields, and potential optimisation methodologies that could be applied to extracting DNA from ICT kit buffers. While it has been demonstrated that sufficient DNA yields can be extracted from the buffer of HemaTraceR and RSID-Semen™ kits, the extraction efficiency and yield are lower than those of conventional extraction methods. There also exist gaps within the research of the influence of frozen storage time on DNA yield from these ICT kit buffers, as well as how the buffer composition influences the DNA extraction process. Further research is required within this field; however, if found promising, laboratories may develop methodologies to extract DNA from ICT kit buffers to act as redundancy when primary samples prove inadequate.
Details
- Title
- Influence of Time on DNA Extraction Yield and Test Outcomes from HemaTrace® and RSID-Semen™ Buffers
- Authors/Creators
- Jordan Leckie
- Contributors
- Marie Rye (Supervisor)Brendan Chapman (Supervisor) - Murdoch University, School of Medical, Molecular and Forensic Sciences
- Awarding Institution
- Murdoch University; Masters by Coursework
- Identifiers
- 991005831946807891
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Resource Type
- Thesis
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