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Investigating barley WRKYs for salinity tolerance by scrutinising CRISPR edited mutants
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Investigating barley WRKYs for salinity tolerance by scrutinising CRISPR edited mutants

Lewis J Price
Honours, Murdoch University
2022
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Abstract

Salinity is a natural and anthropogenic process that presents challenges that plants must overcome with various responses. Salinity imposes a two-phase effect which can be simplified to the initial osmotic challenges and, subsequently, the salinity-specific ion toxicities from continual exposure to sodium and chloride ions. Plant responses can be described as a complex gene network involving osmotic balance, ion transport, anti-oxidant response, and hormone signalling pathways typically mediated by transcription factors. One particular transcription factor mega family, WRKY, is being discovered to be a principal regulator for salinity responses. Here a collection of known salinity-responding WRKYs are categorised, and their molecular pathways are summarised. WRKYs collectively play a part in regulating a plant’s osmotic balance, ion transport response, Anti-oxidant response, and hormone signalling pathways. The hormone signalling pathway is further magnified to illuminate the specific relationship between WRKYs and abscisic acid-signalling due to the proliferation of interaction. Observed trends amongst WRKYs are accented, such as group II WRKYs being enriched as the significant regulators for salinity response. For future research, renaming existing WRKYs with the adopting of a standardised naming system to a based on protein structure are put forward.

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