Thesis
Investigating the best practice techniques for the swabbing of DNA from non-porous surfaces
Masters by Coursework, Murdoch University
2022
Abstract
DNA is one of the most frequently encountered types of biological evidence found at the scene of crimes, whether it is sexual abuse, theft, homicide, or assault. One such form of DNA evidence is touch (trace) DNA, transferred from a person or object onto a surface. Recovering this DNA and matching it to a potential perpetrator is now a ubiquitous tool in forensics, with continuous research aiming to improve the efficiency and efficacy of the analyses and profiling processes. Despite constant improvements within the laboratory, an integral factor in getting the best results possible begins at the very start of the process- the sample collection itself.
Although it is the very first step in the timeline of forensic DNA analysis, there has been less focus recently on the optimisation of DNA collection techniques. A review of current literature shows much in terms of extraction, double swabbing, swab types, and the development of alternative collection methods (tape-lifting, m-vac). Yet, there is a notable lack of literature describing moistening agents and their benefits or pitfalls, as well as any improvement upon the manual technique of individual practitioners. This literature review’s objective is to provide an overview of the currently available research and information and expose the presence of any gaps to provide parameters in which to conduct further research. Additionally, the study design proposed within this review aims to pinpoint the optimal swabbing procedures that are often overlooked and hopes to pave a path to standardise DNA collection between trained and untrained practitioners.
Details
- Title
- Investigating the best practice techniques for the swabbing of DNA from non-porous surfaces
- Authors/Creators
- Emma E Haddleton
- Contributors
- Brendan Chapman (Supervisor) - Murdoch University, School of Medical, Molecular and Forensic Sciences
- Awarding Institution
- Murdoch University; Masters by Coursework
- Identifiers
- 991005556067007891
- Murdoch Affiliation
- School of Medical, Molecular and Forensic Sciences
- Resource Type
- Thesis
Metrics
249 File views/ downloads
341 Record Views