Thesis
The haemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days
Masters by Research, Murdoch University
2023
Abstract
Cold-stored whole blood (CSWB) may be preferrable to blood component therapy when treating haemorrhage in dogs because of its physiologic similarities to shed whole blood and logistical advantages in collection, storage, and administration. Also, breed-related differences in coagulation may affect the haemostatic capacity of CSWB from greyhound (GD) compared to non-greyhound dogs (NGD). To investigate this, we cold-stored whole blood from ten NGD and ten GD at 4 °C for 42 days. We periodically determined the platelet closure time (PCT) (NGD only), manual platelet count (PLT) (GD only), INTEM and EXTEM rotational thromboelastometry (ROTEM) parameters alpha-angle and maximum clot firmness (MCF), prothrombin (PT) and activated partial thromboplastin times (aPTT), fibrinogen concentration (FIB), and coagulation factor activities FII, FV, FVII, FVIII, FIX, FX, FXIII:Ag and von Willebrand’s factor (vWF:Ag). Results from NGD and GD were compared. The PCT for all NGD bags were above the device limit by day (d) 7. The PLT for GD CSWB remained stable. Alpha-angle for INTEM and EXTEM decreased to < 50% of baseline at d38 and d31 for NGD, and d31 and d17 for GD. The INTEM and EXTEM MCF decreased to < 50% of baseline at d42 and d28 for NGD and GD. For NGD, FVIII and vWF:Ag activities reduced to < 50%, and FIB reached 0.982 g/dL during storage. For GD, FVIII, FXIII:Ag, and FV activities, reached 50% during storage and FIB was 0.982 g/dL at baseline. For GD CSWB compared with NGD; alpha-angle and MCF for both INTEM and EXTEM, and FII, FV, FIX, and FXIII:Ag activities were significantly lower overall. vWF:Ag activity for GD CSWB was higher overall compared with NGD CSWB. Viscoelastic parameters of NGD and GD CSWB reduce over 42 days, but most parameters remain stable more than 14 days, supporting the use of CSWB as a haemostatic transfusion product for up to two weeks. GD CSWB compared to NGD has less haemostatic activity but with a similar pattern of decline over time.
Details
- Title
- The haemostatic profile of cold-stored whole blood from non-greyhound and greyhound dogs over 42 days
- Authors/Creators
- James L Cooper
- Contributors
- Claire R. Sharp (Supervisor) - Murdoch University, Centre for Terrestrial Ecosystem Science and Sustainability
- Awarding Institution
- Murdoch University; Masters by Research
- Identifiers
- 991005654368107891
- Murdoch Affiliation
- School of Veterinary Medicine
- Resource Type
- Thesis
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