Journal article
1H NMR spectroscopy of serum reveals unique metabolic fingerprints associated with subtypes of surgically induced osteoarthritis in sheep
Journal of Proteome Research, Vol.11(8), pp.4261-4268
2012
Abstract
Osteoarthritis (OA) is a highly prevalent joint disease. Its slow progressive nature and the correlation between pathological changes and clinical symptoms mean that OA is often well advanced by the time of diagnosis. In the absence of any specific pharmacological treatments, there is a pressing need to develop robust biomarkers for OA. We have adopted a nuclear magnetic resonance (NMR)-based metabolomic strategy to identify molecular responses to surgically induced OA in an animal model. Sheep underwent one of three types of surgical procedure (sham (control), meniscal destabilization, MD or anterior cruciate ligament transaction, ACLT), and for every animal a serum sample was collected both pre- and postoperatively, thus, affording two types of "control" data for comparison. 1D 1H NMR spectra were acquired from each sample at 800 MHz and the digitized spectral data were analyzed using principal components analysis and partial least-squares regression discriminant analysis. Our approach, combined with the study design, allowed us to separate the metabolic responses to surgical intervention from those associated with OA. We were able to identify dimethyl sulfone (DMSO 2) as being increased in MD after 4 weeks, while ACLT-induced OA exhibited increased 3-methylhistidine and decreased branched chain amino acids (BCAAs). The findings are discussed in the context of interpretation of metabolomic results in studies of human disease, and the selection of appropriate "control" data sets.
Details
- Title
- 1H NMR spectroscopy of serum reveals unique metabolic fingerprints associated with subtypes of surgically induced osteoarthritis in sheep
- Authors/Creators
- A.D. Maher (Author/Creator) - Department of Environment, Land, Water and PlanningC. Coles (Author/Creator) - Royal Children's HospitalJ. White (Author/Creator) - Royal Children's HospitalJ.F. Bateman (Author/Creator) - Royal Children's HospitalE.S. Fuller (Author/Creator) - Kolling Institute of Medical ResearchD. Burkhardt (Author/Creator) - Kolling Institute of Medical ResearchC.B. Little (Author/Creator) - Kolling Institute of Medical ResearchM. Cake (Author/Creator) - Murdoch UniversityR. Read (Author/Creator) - Murdoch UniversityM.B. McDonagh (Author/Creator) - Department of Environment, Land, Water and PlanningS.J. Rochfort (Author/Creator) - Department of Environment, Land, Water and Planning
- Publication Details
- Journal of Proteome Research, Vol.11(8), pp.4261-4268
- Publisher
- American Chemical Society
- Identifiers
- 991005542479007891
- Copyright
- © 2012 American Chemical Society
- Murdoch Affiliation
- School of Veterinary and Biomedical Sciences
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.34 Orthopedics
- 1.34.255 Osteoarthritis
- Web Of Science research areas
- Biochemical Research Methods
- ESI research areas
- Biology & Biochemistry