Journal article
2102Ep embryonal carcinoma cells have compromised respiration and shifted bioenergetic profile distinct from H9 human embryonic stem cells
Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1861(8), pp.2146-2154
2017
Abstract
Recent studies have shown that cellular bioenergetics may be involved in stem cell differentiation. Considering that during cancerogenesis cells acquire numerous properties of stem cells, it is possible to assume that the energy metabolism in tumorigenic cells might be differently regulated. The aim of this study was to compare the mitochondrial bioenergetic profile of normal pluripotent human embryonic stem cells (hESC) and relatively nullipotent embryonal carcinoma cells (2102Ep cell line). We examined three parameters related to cellular bioenergetics: phosphotransfer system, aerobic glycolysis, and oxygen consumption. Activities and expression levels of main enzymes that facilitate energy transfer were measured. The oxygen consumption rate studies were performed to investigate the respiratory capacity of cells. 2102Ep cells showed a shift in energy distribution towards adenylate kinase network. The total AK activity was almost 3 times higher in 2102Ep cells compared to hESCs (179.85±5.73 vs 64.39±2.55mU/mg of protein) and the expression of AK2 was significantly higher in these cells, while CK was downregulated. 2102Ep cells displayed reduced levels of oxygen consumption and increased levels of aerobic glycolysis compared to hESCs. The compromised respiration of 2102Ep cells is not the result of increased mitochondrial mass, increased proton leak, and reduced respiratory reserve capacity of the cells or impairment of respiratory chain complexes. Our data showed that the bioenergetic profile of 2102Ep cells clearly distinguishes them from normal hESCs. This should be considered when this cell line is used as a reference, and highlight the importance of further research concerning energy metabolism of stem cells.
Details
- Title
- 2102Ep embryonal carcinoma cells have compromised respiration and shifted bioenergetic profile distinct from H9 human embryonic stem cells
- Authors/Creators
- L. Ounpuu (Author/Creator)A. Klepinin (Author/Creator)M. Pook (Author/Creator)I. Teino (Author/Creator)N. Peet (Author/Creator)K. Paju (Author/Creator)K. Tepp (Author/Creator)V. Chekulayev (Author/Creator)I. Shevchuk (Author/Creator)S. Kõks (Author/Creator)T. Maimets (Author/Creator)T. Kaambre (Author/Creator)
- Publication Details
- Biochimica et Biophysica Acta (BBA) - General Subjects, Vol.1861(8), pp.2146-2154
- Publisher
- Elsevier BV
- Identifiers
- 991005543412907891
- Copyright
- © 2017 Elsevier B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.197 Molecular & Cell Biology - Mitochondria
- 1.197.2067 Creatine Metabolism
- Web Of Science research areas
- Biochemistry & Molecular Biology
- Biophysics
- ESI research areas
- Biology & Biochemistry