A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana

M. Awazuhara; H. Kim; D.B. Goto; A. Matsui; H. Hayashi; M. Chino; S-G Kim; S. Naito; T. Fujiwara

doi:10.1016/S0168-9452(02)00064-X

Back

A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana

Journal article

Peer reviewed

A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana

M. Awazuhara, H. Kim, D.B. Goto, A. Matsui, H. Hayashi, M. Chino, S-G Kim, S. Naito and T. Fujiwara

Plant Science, Vol.163(1), pp.75-82

2002

DOI: https://doi.org/10.1016/S0168-9452(02)00064-X

Files and links (1)

url

Link to Published Version *Subscription may be requiredView

Abstract

The seed-specific promoter of the β subunit gene of β-conglycinin, a major seed storage protein of soybean, is upregulated in response to both sulfur and nitrogen nutrition. This response was studied in non-seed tissues of transgenic Arabidopsis thaliana by fusing the β subunit promoter (Pβ) downstream of an enhancer from the cauliflower mosaic virus 35S RNA promoter (P35S) and using the β-glucuronidase (GUS) reporter gene. The chimeric promoter was upregulated by sulfur deficiency (ΔS), indicating that the Pβ is able to direct the ΔS response in non-seed tissues. Deletion analysis of the Pβ revealed that, in seeds, the -307 - -73 bp region is sufficient for the ΔS response. Insertion of the 235 bp region into the P35S conferred the ΔS response on this constitutive promoter in both seed and non-seed tissues. The extent of upregulation by ΔS was more evident when three of the 235 bp fragments were tandemly inserted into the P35S. The findings presented here suggest that a common mechanism exists for sulfur-regulated expression of the Pβ in both seed and non-seed tissues and that this 235 bp region of the Pβ is sufficient for regulation by both sulfur and nitrogen nutrition.

Details

Title: A 235-bp region from a nutritionally regulated soybean seed-specific gene promoter can confer its sulfur and nitrogen response to a constitutive promoter in aerial tissues of Arabidopsis thaliana
Authors/Creators: M. Awazuhara (Author/Creator)
H. Kim (Author/Creator)
D.B. Goto (Author/Creator)
A. Matsui (Author/Creator)
H. Hayashi (Author/Creator)
M. Chino (Author/Creator)
S-G Kim (Author/Creator)
S. Naito (Author/Creator)
T. Fujiwara (Author/Creator)
Publication Details: Plant Science, Vol.163(1), pp.75-82
Publisher: Elsevier
Identifiers: 991005541200007891
Copyright: © 2002 Elsevier Science Ireland Ltd.
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

#12 Responsible Consumption & Production

Source: InCites

Metrics

38 Record Views

27 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.180 Microbial Biotechnology; 3.180.1184 Amino Acid Biosynthesis
Web Of Science research areas: Biochemistry & Molecular Biology; Plant Sciences
ESI research areas: Plant & Animal Science