A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Li Liu; A. Wang; R. Appels; J. Ma; X. Xia; P. Lan; Z. He; F. Bekes; Y. Yan; W. Ma

doi:10.1016/j.jcs.2009.05.006

Back

A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Journal article

Open access

Peer reviewed

A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding

Li Liu, A. Wang, R. Appels, J. Ma, X. Xia, P. Lan, Z. He, F. Bekes, Y. Yan and W. Ma

Journal of Cereal Science, Vol.50(2), pp.295-301

2009

DOI: https://doi.org/10.1016/j.jcs.2009.05.006

Files and links (2)

pdf

a_MALDI_TOF.pdfDownload View

Author’s Version Open Access

url

Link to Published Version *Subscription may be requiredView

Abstract

High molecular weight glutenin subunits play an important role in determining wheat dough quality as they confer visco-elastic properties to the dough required for mixing and baking performance. In this work, a collection of 103 genotypes of common wheat from 12 countries was used to analyse the composition of HMW-GS by SDS-PAGE and MALDI-TOF-MS. Results indicated that MALDI-TOF technology is suitable for analyzing most HMW-GS alleles. The allelic diversity at Glu-B1 locus include subunits 6+8b*, 7, 7+8, 7+8a*, 7b*+8, 7OE, 7OE+8, 7OE+8a*, 7OE+8b*, 7+9, 13+16, 14+15, 17+18 and 20. The rapid identification of HMW-GS capability of MALDI-TOF-MS is discussed in relation to its value for screening lines in wheat breeding programs, especially in discriminating subunits 7OE, 8a* and 8b* associated with superior quality. A new glutenin subunit 7b*+8 was found in Japanese germplasm Eshimashinriki.

Details

Title: A MALDI-TOF based analysis of high molecular weight glutenin subunits for wheat breeding
Authors/Creators: Li Liu (Author/Creator)
A. Wang (Author/Creator)
R. Appels (Author/Creator)
J. Ma (Author/Creator)
X. Xia (Author/Creator)
P. Lan (Author/Creator)
Z. He (Author/Creator)
F. Bekes (Author/Creator)
Y. Yan (Author/Creator)
W. Ma (Author/Creator)
Publication Details: Journal of Cereal Science, Vol.50(2), pp.295-301
Publisher: Academic Press
Identifiers: 991005543088707891
Copyright: © 2009 Elsevier Ltd.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

This output has contributed to the advancement of the following goals:

Source: InCites

Metrics

526 File views/ downloads

140 Record Views

60 Times Cited - Web of Science

InCites Highlights

These are selected metrics from InCites Benchmarking & Analytics tool, related to this output

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.85 Food Science & Technology; 3.85.99 Cereal Starch Properties
Web Of Science research areas: Food Science & Technology
ESI research areas: Agricultural Sciences