Journal article
A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B
Science, Vol.322(5898), pp.101-104
2008
Abstract
As the staple food for 35% of the world's population, wheat is one of the most important crop species. To date, sequence-based tools to accelerate wheat improvement are lacking. As part of the international effort to sequence the 17–billion–base-pair hexaploid bread wheat genome (2n = 6x = 42 chromosomes), we constructed a bacterial artificial chromosome (BAC)–based integrated physical map of the largest chromosome, 3B, that alone is 995 megabases. A chromosome-specific BAC library was used to assemble 82% of the chromosome into 1036 contigs that were anchored with 1443 molecular markers, providing a major resource for genetic and genomic studies. This physical map establishes a template for the remaining wheat chromosomes and demonstrates the feasibility of constructing physical maps in large, complex, polyploid genomes with a chromosome-based approach.
Details
- Title
- A Physical Map of the 1-Gigabase Bread Wheat Chromosome 3B
- Authors/Creators
- E. Paux (Author/Creator)P. Sourdille (Author/Creator)J. Salse (Author/Creator)C. Saintenac (Author/Creator)F. Choulet (Author/Creator)P. Leroy (Author/Creator)A. Korol (Author/Creator)M. Michalak (Author/Creator)S. Kianian (Author/Creator)W. Spielmeyer (Author/Creator)E. Lagudah (Author/Creator)D. Somers (Author/Creator)A. Kilian (Author/Creator)M. Alaux (Author/Creator)S. Vautrin (Author/Creator)H. Berges (Author/Creator)K. Eversole (Author/Creator)R. Appels (Author/Creator)J. Safar (Author/Creator)H. Simkova (Author/Creator)J. Dolezel (Author/Creator)M. Bernard (Author/Creator)C. Feuillet (Author/Creator)
- Publication Details
- Science, Vol.322(5898), pp.101-104
- Publisher
- American Association for the Advancement of Science
- Identifiers
- 991005544606407891
- Murdoch Affiliation
- School of Information Technology
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.4 Crop Science
- 3.4.96 QTL
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics