A T2 cytokine environment may not limit T1 responses in human immunodeficiency virus patients with a favourable response to antiretroviral therapy

P. Price; N.M. Keane; S. Lee; A.F.Y. Lim; E.J. McKinnon; M.A. French

doi:10.1111/j.1365-2567.2006.02407.x

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A T2 cytokine environment may not limit T1 responses in human immunodeficiency virus patients with a favourable response to antiretroviral therapy

Journal article

Peer reviewed

A T2 cytokine environment may not limit T1 responses in human immunodeficiency virus patients with a favourable response to antiretroviral therapy

P. Price, N.M. Keane, S. Lee, A.F.Y. Lim, E.J. McKinnon and M.A. French

Immunology, Vol.119(1), pp.74-82

2006

DOI: https://doi.org/10.1111/j.1365-2567.2006.02407.x

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Abstract

Low-level production of interferon-γ (IFN-γ) marks human immunodeficiency virus (HIV)-induced immunodeficiency and has been ascribed to a bias towards T2 cytokines. This was investigated in two cross-sectional studies of HIV patients who were immunodeficient when they began antiretroviral therapy (ART) and had stable increases in CD4 T-cell counts. Blood leucocytes were assessed unstimulated or after stimulation with cytomegalovirus (CMV), anti-CD3 or mitogen. IFN-γ and interleukin (IL)-5 responses were initially assessed by enzyme-linked immunosorbent spot-forming cell assay (ELISPOT) and enzyme-linked immunosorbent assay (ELISA). We then adopted a sensitive reverse transcription–polymerase chain reaction (RT–PCR) system to assess IFN-γ, IL-5, IL-4 and IL-4δ2 (an inhibitory splice variant of IL-4) mRNA. The results were correlated with putative serological markers of a T1 [lymphocyte activation gene-3 (LAG-3), CD26] or a T2 [CD30, immunoglobulin E (IgE)] cytokine environment. IL-5 production and IgE levels were elevated in patients. IgE levels did not correlate with IFN-γ, but showed an inverse correlation with IL-5 released in culture (P = 0·05). The levels of IL-4, IFN-γ, IL-5 and IL-4δ2 mRNA were correlated after anti-CD3 stimulation, where IL-5 was the best predictor of IFN-γ mRNA (P = 0·006). Weak positive correlations were evident between CD30 and cytokine mRNA levels, whilst IgE correlated inversely with IL-4, IL-4δ2, IL-5 and IFN-γ mRNA levels. These analyses provide no evidence for an inverse relationship between T1 and T2 cytokine responses in HIV patients, but suggest that the elevation of IgE marks low cytokine responses.

Details

Title: A T2 cytokine environment may not limit T1 responses in human immunodeficiency virus patients with a favourable response to antiretroviral therapy
Authors/Creators: P. Price (Author/Creator) - The University of Western Australia
N.M. Keane (Author/Creator) - Royal Perth Hospital
S. Lee (Author/Creator) - Royal Perth Hospital
A.F.Y. Lim (Author/Creator)
E.J. McKinnon (Author/Creator) - Royal Perth Hospital
M.A. French (Author/Creator) - Royal Perth Hospital
Publication Details: Immunology, Vol.119(1), pp.74-82
Identifiers: 991005541174607891
Copyright: 2006 Blackwell Publishing Ltd
Murdoch Affiliation: Centre for Clinical Immunology and Biomedical Statistics
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 1 Clinical & Life Sciences; 1.66 HIV; 1.66.46 HIV Pathogenesis
Web Of Science research areas: Immunology
ESI research areas: Immunology