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A comparative study of functional assays for tissue factor pathway inhibitor using normal plasma and clinical samples
Journal article   Peer reviewed

A comparative study of functional assays for tissue factor pathway inhibitor using normal plasma and clinical samples

M.J. Adams and R. Oostryck
Blood Coagulation and Fibrinolysis, Vol.11(4), pp.327-333
2000
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Abstract

Tissue factor pathway inhibitor (TFPI) is a Kunitz-type inhibitor that regulates the initiation of tissue factor-mediated coagulation. Recent reports in the literature have described variable results using different methodologies for TFPI measurement. In this study, we used one clotting and two amidolytic methodologies to assess TFPI functional levels. The study groups included normal healthy donors as well as patients with acute hepatitis, diabetes, coronary artery bypass graft operations, deep vein thrombosis, and prior to and during heparin therapy. The aims were to compare the results obtained in normal plasma using different assay systems, to compare TFPI levels in a range of clinical samples, including those previously not determined using a clotting methodology, and to report TFPI levels in patient groups previously not investigated. The results clearly demonstrate poor correlation between functional TFPI values using the different methodologies, highlighting the requirement for standardization.

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Source: InCites

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Citation topics
1 Clinical & Life Sciences
1.75 Blood Clotting
1.75.1434 Coagulation Pathways
Web Of Science research areas
Hematology
ESI research areas
Clinical Medicine
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