Journal article
A complete platelet system for metabolic studies: Pure, defined, and viable over 8 hours
Analytical Biochemistry, Vol.226(1), pp.113-119
1995
Abstract
We have been using a human platelet preparation to investigate various aspects of cellular metabolism. These experiments require a pure, quantitated platelet preparation that is viable for up to 8 h. The purity of the preparation has previously been characterized and we have now assessed its viability over 8 h and developed a simple spectrophotometric counting method. This method of counting results in estimations that are within 6% of those from an electronic cell counter (Coulter). Pure platelets stored in a Hepes physiological saline were resuspended periodically in autologous plasma to assess function over 24 h. The platelets consumed oxygen, changed shape, aggregated, and released dense granule ATP at all times. There was a slow and linear decrease in the various processes with time, ranging from 1.7% per hour for oxygen consumption to 3.8% per hour for percentage aggregation. The preparation is therefore not stable, but the changes with time are small and defined and it represents a useful system for the study of platelet and cell biology.
Details
- Title
- A complete platelet system for metabolic studies: Pure, defined, and viable over 8 hours
- Authors/Creators
- M. Guppy (Author/Creator) - The University of Western AustraliaM.E. Whisson (Author/Creator)M. McConnell (Author/Creator)L. Abas (Author/Creator) - Graduate Research School
- Publication Details
- Analytical Biochemistry, Vol.226(1), pp.113-119
- Publisher
- Academic Press
- Identifiers
- 991005546331507891
- Murdoch Affiliation
- School of Veterinary Studies
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
52 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.75 Blood Clotting
- 1.75.324 Transfusion
- Web Of Science research areas
- Biochemical Research Methods
- Biochemistry & Molecular Biology
- Chemistry, Analytical
- ESI research areas
- Biology & Biochemistry