Journal article
A convex “Ferguson plot” of polystyrene particles in electrophoresis on 0.25 to 2.0 % polyacrylamide (30 % Bis-crosslinked)
Electrophoresis, Vol.8(10), pp.471-476
1987
Abstract
The plot of log(mobility) vs. gel concentration for polystyrene particles of 26.9 and 44.2 nm radius in 30% Bis-crosslinked polyacrylamide below 2.2 %T, containing 22.5% Metrizamide and 6 M urea, is convex. The convex plots of the two particles are essentially parallel. This parallelism suggests a retardation by a mechanism other than sieving. The parallel plots cover a range of polyacrylamide concentrations characterized by progressive fluidity as %T is lowered below 2 %T. The convex curvature renders suspect the previous free mobility and valence determinations in polyacrylamide gel electrophoresis obtained by linear extrapolation of Ferguson plots to 0 %T. Nonlinear extrapolation to 0 % T of plots of log(mobility) vs. gel concentration in the concentration range below 2 %T of 30% Bis-crosslinked polyacrylamide for the two polystyrene particles, corrected for the effects on mobilities of Metrizamide, urea and a lower ionic strength, provides free mobility values similar to those obtained under otherwise identical conditions on agarose gel electrophoresis.
Details
- Title
- A convex “Ferguson plot” of polystyrene particles in electrophoresis on 0.25 to 2.0 % polyacrylamide (30 % Bis-crosslinked)
- Authors/Creators
- L. Orbán (Author/Creator)D. Tietz (Author/Creator)A. Chrambach (Author/Creator)
- Publication Details
- Electrophoresis, Vol.8(10), pp.471-476
- Publisher
- John Wiley & Sons Ltd
- Identifiers
- 991005544279907891
- Copyright
- © 1987 VCH Verlagsgesellschaft mbH
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 2 Chemistry
- 2.166 Chromatography & Electrophoresis
- 2.166.730 Capillary Electrophoresis
- Web Of Science research areas
- Biochemical Research Methods
- Chemistry, Analytical
- ESI research areas
- Chemistry