Journal article
A feasibility study of metabolic phenotyping of dried blood spot specimens in rural Chinese women exposed to household air pollution
Journal of Exposure Science & Environmental Epidemiology
2020
Abstract
Background
Exposure–response studies and policy evaluations of household air pollution (HAP) are limited by current methods of exposure assessment which are expensive and burdensome to participants.
Methods
We collected 152 dried blood spot (DBS) specimens during the heating and non-heating seasons from 53 women who regularly used biomass-burning stoves for cooking and heating. Participants were enrolled in a longitudinal study in China. Untargeted metabolic phenotyping of DBS were generated using ultra-high performance liquid chromatography coupled with mass spectrometry to exemplify measurement precision and assessment for feasibility to detect exposure to HAP, evaluated by season (high pollution vs. low pollution) and measured personal exposure to fine particulate matter <2.5 μm diameters (PM2.5) and black carbon (BC) in the 48-h prior to collecting the DBS specimen.
Results
Metabolites e.g., amino acids, acyl-carnitines, lyso-phosphorylcholines, sphinganine, and choline were detected in the DBS specimens. Our approach is capable of detecting the differences in personal exposure to HAP whilst showing high analytical reproducibility, coefficient of variance (CV) <15%, meeting the U.S. Food and Drug Administration criteria.
Conclusions
Our results provide a proof of principle that high-resolution metabolic phenotypic data can be generated using a simple DBS extraction method thus suitable for exposure studies in remote, low-resource settings where the collection of serum and plasma is logistically challenging or infeasible. The analytical run time (19 min/specimen) is similar to most global phenotyping methods and therefore suitable for large-scale application.
Details
- Title
- A feasibility study of metabolic phenotyping of dried blood spot specimens in rural Chinese women exposed to household air pollution
- Authors/Creators
- R.L. Loo (Author/Creator) - Murdoch UniversityQ. Lu (Author/Creator) - Fudan UniversityE.M. Carter (Author/Creator) - Colorado State UniversityS. Liu (Author/Creator) - Chinese Academy of SciencesS. Clark (Author/Creator) - McGill UniversityY. Wang (Author/Creator) - Nanyang Technological UniversityJ. Baumgartner (Author/Creator) - McGill UniversityH. Tang (Author/Creator) - Chinese Academy of SciencesQ. Chan (Author/Creator) - Imperial College London
- Publication Details
- Journal of Exposure Science & Environmental Epidemiology
- Publisher
- Springer Nature
- Identifiers
- 991005541036707891
- Copyright
- © 2020 Springer Nature Limited
- Murdoch Affiliation
- Australian National Phenome Centre
- Language
- English
- Resource Type
- Journal article
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- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 8 Earth Sciences
- 8.124 Environmental Sciences
- 8.124.552 Air Pollution
- Web Of Science research areas
- Environmental Sciences
- Public, Environmental & Occupational Health
- Toxicology
- ESI research areas
- Environment/Ecology