Journal article
A helicase gene (helO) in Rhizobium meliloti WSM419
FEMS Microbiology Letters, Vol.153(1), pp.43-49
1997
Abstract
A 2.8 kb BamHI DNA fragment adjacent to a BamHI fragment containing actR-actS (a sensor/regulator pair required for low pH tolerance in Rhizobium meliloti WSM419) was cloned and sequenced. A computer predicted protein of 821 amino acids, designated HelO, showed extensive similarity with 'DEAH' motif helicases. Expression of helO was higher at pH 7.0 than pH 5.8 and it did not require the product of the actR gene. Inactivation of helO by insertion of a Ω interposon at codon 40 did not affect nodulation, growth or tolerance to low pH, high temperature, osmolarity or elevated levels of copper or zinc.
Details
- Title
- A helicase gene (helO) in Rhizobium meliloti WSM419
- Authors/Creators
- W.G. Reeve (Author/Creator)R.P. Tiwari (Author/Creator)M.J. Dilworth (Author/Creator)A.R. Glenn (Author/Creator)
- Publication Details
- FEMS Microbiology Letters, Vol.153(1), pp.43-49
- Publisher
- Blackwell Publishing
- Identifiers
- 991005541932607891
- Copyright
- (c) Blackwell Publishing
- Murdoch Affiliation
- Centre for Rhizobium Studies
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.97 Plant Pathology
- 3.97.892 Rhizobium-Legume Symbiosis
- Web Of Science research areas
- Microbiology
- ESI research areas
- Microbiology