A high-resolution HPLC-QqTOF platform using parallel reaction monitoring for in-depth lipid discovery and rapid profiling

D. Yu; T.W.T. Rupasinghe; B.A. Boughton; S.H.A. Natera; C.B. Hill; P. Tarazona; I. Feussner; U. Roessner

doi:10.1016/j.aca.2018.03.062

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A high-resolution HPLC-QqTOF platform using parallel reaction monitoring for in-depth lipid discovery and rapid profiling

Journal article

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A high-resolution HPLC-QqTOF platform using parallel reaction monitoring for in-depth lipid discovery and rapid profiling

D. Yu, T.W.T. Rupasinghe, B.A. Boughton, S.H.A. Natera, C.B. Hill, P. Tarazona, I. Feussner and U. Roessner

Analytica Chimica Acta, Vol.1026, pp.87-100

2018

DOI: https://doi.org/10.1016/j.aca.2018.03.062

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Abstract

Here, we developed a robust lipidomics workflow merging both targeted and untargeted approaches on a single liquid chromatography coupled to quadrupole-time of flight (LC-QqTOF) mass spectrometry platform with parallel reaction monitoring (PRM). PRM assays integrate both untargeted profiling from MS1 scans and targeted profiling obtained from MS/MS data. This workflow enabled the discovery of more than 2300 unidentified features and identification of more than 600 lipid species from 23 lipid classes at the level of fatty acid/long chain base/sterol composition in a barley root extracts. We detected the presence of 142 glycosyl inositol phosphorylceramides (GIPC) with HN(Ac)-HA as the core structure of the polar head, 12 cardiolipins and 17 glucuronosyl diacylglycerols (GlcADG) which have been rarely reported previously for cereal crops. Using a scheduled algorithm with up to 100 precursors multiplexed per duty cycle, the PRM assay was able to achieve a rapid profiling of 291 species based on MS/MS data by a single injection. We used this novel approach to demonstrate the applicability and efficiency of the workflow to study salt stress induced changes in the barley root lipidome. Results show that 221 targeted lipids and 888 unknown features were found to have changed significantly in response to salt stress. This combined targeted and untargeted single workflow approach provides novel applications of lipidomics addressing biological questions.

Details

Title: A high-resolution HPLC-QqTOF platform using parallel reaction monitoring for in-depth lipid discovery and rapid profiling
Authors/Creators: D. Yu (Author/Creator) - The University of Melbourne
T.W.T. Rupasinghe (Author/Creator)
B.A. Boughton (Author/Creator) - The University of Melbourne
S.H.A. Natera (Author/Creator) - The University of Melbourne
C.B. Hill (Author/Creator) - Murdoch University
P. Tarazona (Author/Creator) - University of Göttingen
I. Feussner (Author/Creator) - University of Göttingen
U. Roessner (Author/Creator) - The University of Melbourne
Publication Details: Analytica Chimica Acta, Vol.1026, pp.87-100
Publisher: Elsevier
Identifiers: 991005545283107891
Copyright: © 2018 Published by Elsevier B.V.
Murdoch Affiliation: Western Barley Genetics Alliance
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.211 Mass Spectrometry; 2.211.990 Metabolomics
Web Of Science research areas: Chemistry, Analytical
ESI research areas: Chemistry