A locus on barley chromosome 5H affects adult plant resistance to powdery mildew

S. Gupta; E. Vassos; B. Sznajder; R. Fox; K.H.P. Khoo; R. Loughman; K.J. Chalmers; D.E. Mather

doi:10.1007/s11032-018-0858-2

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A locus on barley chromosome 5H affects adult plant resistance to powdery mildew

Journal article

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A locus on barley chromosome 5H affects adult plant resistance to powdery mildew

S. Gupta, E. Vassos, B. Sznajder, R. Fox, K.H.P. Khoo, R. Loughman, K.J. Chalmers and D.E. Mather

Molecular Breeding, Vol.38(8)

2018

DOI: https://doi.org/10.1007/s11032-018-0858-2

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Abstract

Adult plant resistance against plant pathogens is of interest as a means to achieve durable resistance. Prior to this research, the barley lines CLE210 (from Uruguay) and Denar (from the Czech Republic) had been reported to exhibit adult-plant resistance against powdery mildew. Here, populations of doubled haploid lines from crosses of these lines with the susceptible cultivar Baudin were evaluated for powdery mildew resistance in field experiments. Using linkage maps constructed from genotyping-by-sequencing (GBS) data, it was determined that differences in resistance were largely attributable to a region on the long arm of chromosome 5H (5HL). Therefore, KASP™ assays were developed based on GBS tag sequences mapped on that chromosome, providing more reliable genetic maps. In each population, a large-effect QTL was mapped on 5HL. As no sequence variation was detected between CLE210 and Denar in this region of 5HL, the two sources of resistance may be identical by descent in the QTL region and carry the same resistance gene. Marker assays from the QTL region were evaluated on a panel of barley lines, providing information that breeders could use to select assays for use in marker-assisted selection.

Details

Title: A locus on barley chromosome 5H affects adult plant resistance to powdery mildew
Authors/Creators: S. Gupta (Author/Creator) - Department of Primary Industries and Regional Development
E. Vassos (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
B. Sznajder (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
R. Fox (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
K.H.P. Khoo (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
R. Loughman (Author/Creator) - Department of Primary Industries and Regional Development
K.J. Chalmers (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
D.E. Mather (Author/Creator) - School of Agriculture, Food and Wine, Waite Research Institute, PMB 1, Glen Osmond, SA 5064 Australia.
Publication Details: Molecular Breeding, Vol.38(8)
Publisher: Kluwer Academic Publishers
Identifiers: 991005539971907891
Copyright: © The Author(s) 2018
Murdoch Affiliation: School of Veterinary and Life Sciences
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Plant & Animal Science