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A low-spin iron complex in human melanoma and rat hepatoma cells and a high-spin iron(II) complex in rat hepatoma cells
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A low-spin iron complex in human melanoma and rat hepatoma cells and a high-spin iron(II) complex in rat hepatoma cells

T.G. St Pierre, D.R. Richardson, E. Baker and J. Webb
Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Vol.1135(2), pp.154-158
1992
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Abstract

Human melanoma and rat hepatoma cells cultured in the presence of low concentrations (2.5 μM) of low-molecular-weight iron (Fe) chelates and Fe-transferrin complexes have been studied with 57Fe Mössbauer spectroscopy. The spectra show that holoferritin is only a minor fraction of the total iron present in the cells. The major form of Fe was in a low-spin state unlike the high-spin Fe(III) found in ferritin. Only about 10% of the Fe could be attributed to ferritin. In addition, the hepatoma cells had a high-spin Fe(II) spectral component which made up about 20% of the Fe present.

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