A metabolite array technology for precision medicine

G. Xie; L. Wang; T. Chen; K. Zhou; Z. Zhang; J. Li; B. Sun; Y. Guo; X. Wang; Y. Wang; H. Zhang; P. Liu; J.K. Nicholson; W. Ge; W. Jia

doi:10.1021/acs.analchem.0c04686

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A metabolite array technology for precision medicine

Journal article

Peer reviewed

A metabolite array technology for precision medicine

G. Xie, L. Wang, T. Chen, K. Zhou, Z. Zhang, J. Li, B. Sun, Y. Guo, X. Wang, Y. Wang, …

Analytical Chemistry, Vol.93(14), pp.5709-5717

2021

DOI: https://doi.org/10.1021/acs.analchem.0c04686

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Abstract

The application of metabolomics in translational research suffers from several technological bottlenecks, such as data reproducibility issues and the lack of standardization of sample profiling procedures. Here, we report an automated high-throughput metabolite array technology that can rapidly and quantitatively determine 324 metabolites including fatty acids, amino acids, organic acids, carbohydrates, and bile acids. Metabolite identification and quantification is achieved using the Targeted Metabolome Batch Quantification (TMBQ) software, the first cross-vendor data processing pipeline. A test of this metabolite array was performed by analyzing serum samples from patients with chronic liver disease (N = 1234). With high detection efficiency and sensitivity in serum, urine, feces, cell lysates, and liver tissue samples and suitable for different mass spectrometry systems, this metabolite array technology holds great potential for biomarker discovery and high throughput clinical testing. Additionally, data generated from such standardized procedures can be used to generate a clinical metabolomics database suitable for precision medicine in next-generation healthcare.

Details

Title: A metabolite array technology for precision medicine
Authors/Creators: G. Xie (Author/Creator) - Shanghai Jiao Tong University
L. Wang (Author/Creator) - Human Metabolomics Institute, Inc., Shenzhen, Guangdong 518109, China.
T. Chen (Author/Creator) - Shanghai Jiao Tong University
K. Zhou (Author/Creator) - Human Metabolomics Institute, Inc., Shenzhen, Guangdong 518109, China.
Z. Zhang (Author/Creator) - Nanjing Drum Tower Hospital
J. Li (Author/Creator) - Hong Kong Baptist University
B. Sun (Author/Creator) - Nanjing Drum Tower Hospital
Y. Guo (Author/Creator) - Hong Kong Baptist University
X. Wang (Author/Creator) - Shanghai University of Traditional Chinese Medicine
Y. Wang (Author/Creator) - Shanghai University of Traditional Chinese Medicine
H. Zhang (Author/Creator) - Shanghai University of Traditional Chinese Medicine
P. Liu (Author/Creator) - Shanghai University of Traditional Chinese Medicine
J.K. Nicholson (Author/Creator) - Australian National Phenome Center, Health Futures Institute, Murdoch University, Perth, WA 6150, Australia.
W. Ge (Author/Creator) - Nanjing Drum Tower Hospital
W. Jia (Author/Creator) - Hong Kong Baptist University
Publication Details: Analytical Chemistry, Vol.93(14), pp.5709-5717
Publisher: American Chemical Society
Identifiers: 991005542876307891
Copyright: © 2021 American Chemical Society
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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199 Times Cited - Web of Science

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Highly Cited Paper

Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.211 Mass Spectrometry; 2.211.990 Metabolomics
Web Of Science research areas: Chemistry, Analytical
ESI research areas: Chemistry