Journal article
A metabolomic approach to dissecting osmotic stress in the wheat pathogen Stagonospora nodorum
Fungal Genetics and Biology, Vol.45(11), pp.1479-1486
2008
Abstract
A non-targeted metabolomics approach was used to identify significant changes in metabolism upon exposure of the wheat pathogen Stagonospora nodorum to 0.5 M NaCl. The polyol arabitol, and to a lesser extent glycerol, was found to accumulate in response to the osmotic stress treatment. Amino acid synthesis was strongly down-regulated whilst mannitol levels were unaffected. A reverse genetic approach was undertaken to dissect the role of arabitol metabolism during salt stress. Strains of S. nodorum lacking a gene encoding an l-arabitol dehydrogenase (abd1), a xylitol dehydrogenase (xdh1) and a double-mutant lacking both genes (abd1xdh1) were exposed to salt and the intracellular metabolites analysed. Arabitol levels were significantly up-regulated upon salt stress in the xdh1 strains but were significantly lower than the wild-type. Arabitol was not significantly different in either the abd1 or the abd1xdh1 strains during osmotic stress but the concentration of glycerol was significantly higher indicating a compensatory mechanism in operation. Genome sequence analysis identified a second possible enzyme capable of synthesizing arabitol explaining the basal level of arabitol present in the abd1xdh1 strains. This study identified that arabitol is the primary compatible solute in S. nodorum but in-built levels of redundancy are present allowing the fungus to tolerate osmotic stress.
Details
- Title
- A metabolomic approach to dissecting osmotic stress in the wheat pathogen Stagonospora nodorum
- Authors/Creators
- R.G.T. Lowe (Author/Creator) - Murdoch UniversityM. Lord (Author/Creator) - Murdoch UniversityK. Rybak (Author/Creator) - Murdoch UniversityR.D. Trengove (Author/Creator) - Murdoch UniversityR.P. Oliver (Author/Creator) - Murdoch UniversityP.S. Solomon (Author/Creator) - Murdoch University
- Publication Details
- Fungal Genetics and Biology, Vol.45(11), pp.1479-1486
- Publisher
- Elsevier
- Identifiers
- 991005544579607891
- Copyright
- 2008 Elsevier Inc.
- Murdoch Affiliation
- School of Pharmacy
- Language
- English
- Resource Type
- Journal article
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- Citation topics
- 1 Clinical & Life Sciences
- 1.148 Medical Mycology
- 1.148.240 Saccharomyces Cerevisiae
- Web Of Science research areas
- Genetics & Heredity
- Mycology
- ESI research areas
- Plant & Animal Science