Journal article
A metataxonomic tool to investigate the diversity of treponema
Frontiers in Microbiology, Vol.10, Article 2094
2019
Abstract
The genus Treponema contains a number of human and animal pathogenic as well as symbiotic bacteria that are found in vastly different anatomical and environmental habitats. Our understanding of the species range, evolution, and biology of these important bacteria is still limited. To explore the diversity of treponemes, we established, validated, and tested a novel metataxonomic approach. As the informative nature of the hypervariable regions of the 16S rRNA gene differ, we first analyzed each variable region independently. Considering the in silico results obtained, we established and validated the sequencing of the V4-region of the 16S rRNA gene using known mixtures of Treponema species as well as a selected number of clinical samples. The metataxonomic approach was able to identify Treponema to a near-species level. We demonstrate that using a spirochete-specific enrichment, our method is applicable to complex microbial communities and large variety of biological samples. The metataxonomic approach described provides a useful method to unravel the full diversity and range of Treponema in various ecosystems.
Details
- Title
- A metataxonomic tool to investigate the diversity of treponema
- Authors/Creators
- L.K. Hallmaier-Wacker (Author/Creator) - German Primate CenterS. Lüert (Author/Creator) - German Primate CenterS. Gronow (Author/Creator) - Leibniz Institute DSMZ – German Collection of Microorganisms and Cell CulturesC. Spröer (Author/Creator) - Leibniz Institute DSMZ – German Collection of Microorganisms and Cell CulturesJ. Overmann (Author/Creator) - Leibniz Institute DSMZ – German Collection of Microorganisms and Cell CulturesN. Buller (Author/Creator) - Department of Primary Industries and Regional DevelopmentR.J. Vaughan-Higgins (Author/Creator) - Murdoch UniversityS. Knauf (Author/Creator) - German Primate Center
- Publication Details
- Frontiers in Microbiology, Vol.10, Article 2094
- Publisher
- Frontiers
- Identifiers
- 991005541179307891
- Copyright
- © 2019 the Author(s).
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
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