A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat

M. Prasad; R.K. Varshney; A. Kumar; H.S. Balyan; P.C. Sharma; K.J. Edwards; M. Harjit-Singh; H.S. Dhaliwal; J.K. Roy; P.K. Gupta

doi:10.1007/s001220051242

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A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat

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A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat

M. Prasad, R.K. Varshney, A. Kumar, H.S. Balyan, P.C. Sharma, K.J. Edwards, M. Harjit-Singh, H.S. Dhaliwal, J.K. Roy and P.K. Gupta

Theoretical and Applied Genetics, Vol.99(1-2), pp.341-345

1999

DOI: https://doi.org/10.1007/s001220051242

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Abstract

This study was undertaken with a view to tag gene(s) controlling grain protein content (GPC) using molecular markers in bread wheat. For this purpose, the genotype PH132 with high protein content (13.5%) was crossed with genotype WL711 with significantly lower protein content (9.7%), and 100 RILs were derived. These RILs showed normal distribution for protein content. The parental genotypes were analysed with 232 STMS primer pairs for detection of polymorphism. Of these, 167 primer pairs gave scorable amplification products, and 57 detected polymorphism between the parents. Using each of these 57 primer pairs, we carried out bulked segregant analysis on RILs representing the two extremes of the distribution. One primer pair for the locus wmc41 showed association with protein content. This was further confirmed through selective genotyping. The co-segregation data on the molecular marker (wmc41) and protein content on 100 RILs was analysed by means of a single-marker linear regression approach. Significant regression suggested linkage between wmc41 and a QTL (designated as QGpc.ccsu-2D.) for protein content. The results showed that this marker-linked QTL accounted for 18.73% of the variation for protein content between the parents. The marker has been located on chromosome arm 2DL using nulli-tetrasomic lines and two ditelocentric stocks for chromosome 2D.

Details

Title: A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat
Authors/Creators: M. Prasad (Author/Creator) - Chaudhary Charan Singh University
R.K. Varshney (Author/Creator) - Chaudhary Charan Singh University
A. Kumar (Author/Creator) - Chaudhary Charan Singh University
H.S. Balyan (Author/Creator) - Chaudhary Charan Singh University
P.C. Sharma (Author/Creator) - Chaudhary Charan Singh University
K.J. Edwards (Author/Creator) - University of Bristol
M. Harjit-Singh (Author/Creator)
H.S. Dhaliwal (Author/Creator) - Biotechnology Centre, P. A. U., Ludhiana-141004, India, , IN
J.K. Roy (Author/Creator) - Chaudhary Charan Singh University
P.K. Gupta (Author/Creator) - Chaudhary Charan Singh University
Publication Details: Theoretical and Applied Genetics, Vol.99(1-2), pp.341-345
Publisher: Springer Verlag
Identifiers: 991005541189807891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.4 Crop Science; 3.4.96 QTL
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Agricultural Sciences