A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection

D. Li; I. Barclay; K. Jose; K. Stefanova; R. Appels

doi:10.1007/s11032-008-9168-4

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A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection

Journal article

Peer reviewed

A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection

D. Li, I. Barclay, K. Jose, K. Stefanova and R. Appels

Molecular Breeding, Vol.22(2), pp.217-225

2008

DOI: https://doi.org/10.1007/s11032-008-9168-4

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Abstract

A new mutation at the acetohydroxyacid synthase (AHAS) locus on chromosome 6D of wheat was analyzed in detail because it conferred an improved resistance to the imidazolinone group of herbicides. Sequence analysis showed that the mutation was at the Ala122 position (A122T), a position in AHAS which has not to date been identified in imidazolinone resistant wheat lines even though the position has been identified in other plants and is associated with resistance. An allele-specific assay for the mutation (in the wheat line Brookton-8) was developed and used in a genetic analysis. Two mapping populations were analysed and the doubled haploid progeny from the cross Brookton-8 × Clearfield STL proved to be most informative. The AHASAla122 mutation (A122T) was allelic to the AHASSer653 mutation (S653N) in Clearfield STL (Imi1, on chromosome 6D) and hence was assigned to the chromosome 6D locus. The analysis of the doubled haploid lines in the mapping population demonstrated the greater resistance conferred by the A122T mutation because lines from the same cross and carrying either the A122T or S653N mutations could be directly compared.

Details

Title: A mutation at the Ala122 position of acetohydroxyacid synthase (AHAS) located on chromosome 6D of wheat: improved resistance to imidazolinone and a faster assay for marker assisted selection
Authors/Creators: D. Li (Author/Creator) - Murdoch University
I. Barclay (Author/Creator) - Agriculture and Food
K. Jose (Author/Creator) - Agriculture and Food
K. Stefanova (Author/Creator) - Agriculture and Food
R. Appels (Author/Creator) - Murdoch University
Publication Details: Molecular Breeding, Vol.22(2), pp.217-225
Publisher: Kluwer Academic Publishers
Identifiers: 991005540964507891
Copyright: 2008 Springer Science + Business Media B.V.
Murdoch Affiliation: State Agricultural Biotechnology Centre
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration
Citation topics: 3 Agriculture, Environment & Ecology; 3.275 Crop Protection; 3.275.705 Herbicide Resistance
Web Of Science research areas: Agronomy; Genetics & Heredity; Horticulture; Plant Sciences
ESI research areas: Plant & Animal Science