Journal article
A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley
Functional & Integrative Genomics, Vol.11(1), pp.103-110
2011
Abstract
Low phytic acid grains can provide a solution to dietary micronutrient deficiency and environmental pollution. A low phytic acid 1-1 (lpa1-1) barley mutant was identified using forward genetics and the mutant gene was mapped to chromosome 2HL. Comparative genomic analysis revealed that the lpa1-1 gene was located in the syntenic region of the rice Os-lpa-MH86-1 gene on chromosome 4. The gene ortholog of rice Os-lpa-MH86-1 (designated as HvST) was isolated from barley using polymerase chain reaction and mapped to chromosome 2HL in a doubled haploid population of Clipper×Sahara. The results demonstrate the collinearity between the rice Os-lpa-MH86-1 gene and the barley lpa1-1 region. Sequence analysis of HvST revealed a single base pair substitution (C→T transition) in the last exon of the gene in lpa1-1 (M422), which resulted in a nonsense mutation. These results will facilitate our understanding of the molecular mechanisms controlling the low phytic acid phenotype and assist in the development of a diagnostic marker for the selection of the lpa1-1 gene in barley.
Details
- Title
- A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley
- Authors/Creators
- H. Ye (Author/Creator)X-Q Zhang (Author/Creator)S. Broughton (Author/Creator)S. Westcott (Author/Creator)D. Wu (Author/Creator)R. Lance (Author/Creator)C. Li (Author/Creator)
- Publication Details
- Functional & Integrative Genomics, Vol.11(1), pp.103-110
- Publisher
- Springer Verlag
- Identifiers
- 991005544684507891
- Copyright
- © 2011 Springer-Verlag.
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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