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A note on hydrolysis of tributyrin by Branhamella and Neisseria
Journal article   Peer reviewed

A note on hydrolysis of tributyrin by Branhamella and Neisseria

T.V. Riley
Journal of Applied Bacteriology, Vol.62(6), pp.539-542
2008
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Abstract

Sixty‐three strains of Branhamella and Neisseria were tested by two methods for their ability to hydrolyse glycerol tributyrate. After the conventional plate test, gas liquid chromatographical (GLC) analysis of the agar medium was carried out to detect the hydrolysis product, butyric acid, and other volatile fatty acids. All strains of Branhamella catarrhalis, Neisseria caviae, N. cuniculi and N. ovis but no other Neisseria spp. gave positive results with the conventional test. With GLC, however, most strains of Branhamella and Neisseria were shown to liberate butyric acid. In addition, some strains liberated acetic and isovaleric acids. Greater amounts of butyric acid were produced by clinical strains, in particular B. catarrhalis, compared with reference strains. It was concluded that the conventional plate test for tributyrin hydrolysis differentiates B. catarrhalis, N. caviae, N. cuniculi and N. ovis from other Neisseria.

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Citation topics
1 Clinical & Life Sciences
1.23 Antibiotics & Antimicrobials
1.23.347 Streptococcus Pneumoniae
Web Of Science research areas
Biotechnology & Applied Microbiology
Microbiology
ESI research areas
Biology & Biochemistry
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