A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit

E. Ingley; J.H. Williams; C.E. Walker; S. Tsai; S. Colley; M.S. Sayer; P.A. Tilbrook; M. Sarna; J.G. Beaumont; S.P. Klinken

doi:10.1016/S0014-5793(99)01188-6

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A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit

Journal article

Peer reviewed

A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit

E. Ingley, J.H. Williams, C.E. Walker, S. Tsai, S. Colley, M.S. Sayer, P.A. Tilbrook, M. Sarna, J.G. Beaumont and S.P. Klinken

FEBS Letters, Vol.459(1), pp.69-74

1999

DOI: https://doi.org/10.1016/S0014-5793(99)01188-6

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Abstract

We report here the isolation of a new member of the ADP-ribosylation factor (ARF)-like family (ARL-6) present in the J2E erythroleukemic cell line, but not its myeloid variants. Consistent with this lineage-restricted expression, ARL-6 mRNA increased with erythropoietin-induced maturation of J2E cells, and decreased with interleukin 6-induced differentiation of M1 monoblastoid cells. In tissues, ARL-6 mRNA was most abundant in brain and kidney. While ARL-6 protein was predominantly cytosolic, its membrane association increased following exposure to GTP-γS, like many members of the ARF/ARL family. Using the yeast two-hybrid system, six molecules which interact with ARL-6 were identified including SEC61β, a subunit of the heterotrimeric protein conducting channel SEC61p. Co-immunoprecipitation of ARL-6 confirmed a stable association between ARL-6 and SEC61β in COS cells. These results demonstrate that ARL-6, a novel member of the ADP-ribosylation factor-like family, interacts with the SEC61β subunit.

Details

Title: A novel ADP-ribosylation like factor (ARL-6), interacts with the protein-conducting channel SEC61β subunit
Authors/Creators: E. Ingley (Author/Creator) - Royal Perth Hospital
J.H. Williams (Author/Creator) - Royal Perth Hospital
C.E. Walker (Author/Creator) - Royal Perth Hospital
S. Tsai (Author/Creator) - Icahn School of Medicine at Mount Sinai
S. Colley (Author/Creator) - Royal Perth Hospital
M.S. Sayer (Author/Creator) - Royal Perth Hospital
P.A. Tilbrook (Author/Creator) - Royal Perth Hospital
M. Sarna (Author/Creator) - Royal Perth Hospital
J.G. Beaumont (Author/Creator) - Royal Perth Hospital
S.P. Klinken (Author/Creator) - Royal Perth Hospital
Publication Details: FEBS Letters, Vol.459(1), pp.69-74
Publisher: Wiley
Identifiers: 991005542582407891
Murdoch Affiliation: Murdoch University
Language: English
Resource Type: Journal article

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 1 Clinical & Life Sciences; 1.96 Cell Biology; 1.96.302 Membrane Trafficking
Web Of Science research areas: Biochemistry & Molecular Biology; Biophysics; Cell Biology
ESI research areas: Biology & Biochemistry