Journal article
A novel chimeric Low-Molecular-Weight glutenin subunit gene from the wild relatives of wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci
Genetics, Vol.180(1), pp.93-101
2008
Abstract
Four LMW-m and one novel chimeric (between LMW-i and LMW-m types) low-molecular-weight glutenin subunit (LMW-GS) genes from Aegilops neglecta (UUMM), Ae. kotschyi (UUSS), and Ae. juvenalis (DDMMUU) were isolated and characterized. Sequence structures showed that the 4 LMW-m-type genes, assigned to the M genome of Ae. neglecta, displayed a high homology with those from hexaploid common wheat. The novel chimeric gene, designed as AjkLMW-i, was isolated from both Ae. kotschyi and Ae. juvenalis and shown to be located on the U genome. Phylogentic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. A total of 20 single nucleotide polymorphisms (SNPs) were detected among the 4 LMW-m genes, with 13 of these being nonsynonymous SNPs that resulted in amino acid substitutions in the deduced mature proteins. Phylogenetic analysis demonstrated that it had higher identity to the LMW-m-type than the LMW-i-type genes. The divergence time estimation showed that the M and D genomes were closely related and diverged at 5.42 million years ago (MYA) while the differentiation between the U and A genomes was 6.82 MYA. We propose that, in addition to homologous recombination, an illegitimate recombination event on the U genome may have occurred 6.38 MYA and resulted in the generation of the chimeric gene AjkLMW-i, which may be an important genetic mechanism for the origin and evolution of LMW-GS Glu-3 alleles as well as other prolamin genes.
Details
- Title
- A novel chimeric Low-Molecular-Weight glutenin subunit gene from the wild relatives of wheat Aegilops kotschyi and Ae. juvenalis: Evolution at the Glu-3 Loci
- Authors/Creators
- X. Li (Author/Creator)W. Ma (Author/Creator)L. Gao (Author/Creator)Y. Zhang (Author/Creator)A. Wang (Author/Creator)K. Ji (Author/Creator)K. Wang (Author/Creator)R. Appels (Author/Creator)Y. Yan (Author/Creator)
- Publication Details
- Genetics, Vol.180(1), pp.93-101
- Publisher
- Genetics Society of America
- Identifiers
- 991005544490407891
- Copyright
- 2008 Genetics Society of America
- Murdoch Affiliation
- State Agricultural Biotechnology Centre
- Language
- English
- Resource Type
- Journal article
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- Domestic collaboration
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- Citation topics
- 3 Agriculture, Environment & Ecology
- 3.85 Food Science & Technology
- 3.85.99 Cereal Starch Properties
- Web Of Science research areas
- Genetics & Heredity
- ESI research areas
- Molecular Biology & Genetics