Journal article
A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs
Forensic Science International: Genetics, Vol.17, pp.91-98
2015
Abstract
A multiplex polymerase chain reaction (PCR) assay (RM-Yplex) was developed which is capable of simultaneously amplifying 13 recently introduced rapidly mutating Y-STR markers (RM Y-STRs). This multiplex assay is expected to aid human identity testing in forensic and other applications to improve differentiating unrelated males and allow separating related males. The 13 RM Y-STR markers included in the multiplex are: DYF387S1, DYF399S1, DYF403S1ab, DYF404S1, DYS449, DYS518, DYS526ab, DYS547, DYS570, DYS576, DYS612, DYS626 and DYS627. This study reflects the proof of concept to analyse all currently known RM Y-STRs simultaneously and describes the optimization of the multiplex assay. The RM-Yplex assay generated complete RM Y-STR profiles down to 62.5 pg of male template DNA, and from male–female DNA mixtures at all ratios tested. We herewith introduce and make available for widespread use in forensic and anthropological studies, an effective and sensitive single multiplex assay for simultaneous genotyping of 13 RM Y-STRs.
Details
- Title
- A novel multiplex assay for simultaneously analysing 13 rapidly mutating Y-STRs
- Authors/Creators
- R. Alghafri (Author/Creator) - University of LancashireW. Goodwin (Author/Creator) - University of LancashireA. Ralf (Author/Creator) - Erasmus University RotterdamM. Kayser (Author/Creator) - Erasmus University RotterdamS. Hadi (Author/Creator) - University of Lancashire
- Publication Details
- Forensic Science International: Genetics, Vol.17, pp.91-98
- Publisher
- Elsevier BV
- Identifiers
- 991005541782207891
- Copyright
- © 2015 Published by Elsevier B.V.
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
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- 1.189 Genome Studies
- 1.189.310 Population Genetics
- Web Of Science research areas
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