A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat

Settasart Sonna; Chatchawan Srisawat; Sakda Jampasa; Orawon Chailapakul; David J Hampson; Nuvee Prapasarakul

doi:10.1080/01652176.2025.2591482

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A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat

Journal article

Open access

Peer reviewed

A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat

Settasart Sonna, Chatchawan Srisawat, Sakda Jampasa, Orawon Chailapakul, David J Hampson and Nuvee Prapasarakul

The Veterinary quarterly, Vol.45(1), 2591482

2025

DOI: https://doi.org/10.1080/01652176.2025.2591482

PMID: 41287526

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Abstract

Animals

Aptamers, Nucleotide - chemistry

Biosensing Techniques - methods

Biosensing Techniques - veterinary

Chickens

Electrochemical Techniques - methods

Electrochemical Techniques - veterinary

Food Microbiology - methods

Graphite - chemistry

Limit of Detection

Meat - microbiology

Nanocomposites - chemistry

Proof of Concept Study

Salmonella typhimurium - isolation & purification

Serum Albumin, Bovine - chemistry

Salmonella Typhimurium (ST) is an important food-borne pathogen, particularly in chicken meat, making its rapid detection essential for food safety. Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. We developed a rapid, ultrasensitive, and portable electrochemical aptasensor platform for detecting ST in samples extracted from chicken meat. The sensor employed graphene oxide–bovine serum albumin (GO-BSA) nanocomposite to enhance electrode biocompatibility, stability, and aptamer immobilization. A specific NH2-modified DNA aptamer targeting ST allowed direct binding without prior extraction. Fabrication steps included GO-BSA drop-casting, aptamer immobilization, and BSA blocking before ST binding. The sensor operates on a signal-off mechanism, measured via differential pulse voltammetry (DPV), where the aptamer-ST complex formation reduces the redox signal of [Fe(CN)6]³-/4- due to insulation and electrostatic repulsion. The aptasensor demonstrated excellent sensitivity (limit of detection 3 CFU/mL) and high specificity. Performance validation using spiked samples extracted from chicken meat confirmed the effective detection of ST in a complex matrix, aligning with the results obtained through culture-based methods. The sensor also maintained good stability for up to 28 days at 4 °C. These results showed that aptasensors can be a rapid, cost-effective, and field-deployable tool for monitoring foodborne pathogens.

Details

Title: A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat
Authors/Creators: Settasart Sonna - Chulalongkorn University
Chatchawan Srisawat - Siriraj Hospital
Sakda Jampasa - Walailak University
Orawon Chailapakul - Chulalongkorn University
David J Hampson - School of Veterinary Medicine, Murdoch University, Perth, Australia
Nuvee Prapasarakul - Chulalongkorn University
Publication Details: The Veterinary quarterly, Vol.45(1), 2591482
Publisher: Informa UK limited, trading as Taylor & Francis Group
Number of pages: 17
Identifiers: 991005833798907891
Murdoch Affiliation: School of Veterinary Medicine
Language: English
Resource Type: Journal article

UN Sustainable Development Goals (SDGs)

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#12 Responsible Consumption & Production

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Collaboration types: Domestic collaboration; International collaboration
Citation topics: 2 Chemistry; 2.145 Biosensors; 2.145.243 Nanobiosensors
Web Of Science research areas: Veterinary Sciences
ESI research areas: Plant & Animal Science