Files and links (1)
CC BY-NC V4.0, Open Access
Journal article
A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat
The Veterinary quarterly, Vol.45(1), 2591482
2025
PMID: 41287526
Abstract
Salmonella Typhimurium (ST) is an important food-borne pathogen, particularly in chicken meat, making its rapid detection essential for food safety. Conventional detection methods are slow and equipment-intensive, creating the need for sensitive and portable alternatives. We developed a rapid, ultrasensitive, and portable electrochemical aptasensor platform for detecting ST in samples extracted from chicken meat. The sensor employed graphene oxide–bovine serum albumin (GO-BSA) nanocomposite to enhance electrode biocompatibility, stability, and aptamer immobilization. A specific NH2-modified DNA aptamer targeting ST allowed direct binding without prior extraction. Fabrication steps included GO-BSA drop-casting, aptamer immobilization, and BSA blocking before ST binding. The sensor operates on a signal-off mechanism, measured via differential pulse voltammetry (DPV), where the aptamer-ST complex formation reduces the redox signal of [Fe(CN)6]³-/4- due to insulation and electrostatic repulsion. The aptasensor demonstrated excellent sensitivity (limit of detection 3 CFU/mL) and high specificity. Performance validation using spiked samples extracted from chicken meat confirmed the effective detection of ST in a complex matrix, aligning with the results obtained through culture-based methods. The sensor also maintained good stability for up to 28 days at 4 °C. These results showed that aptasensors can be a rapid, cost-effective, and field-deployable tool for monitoring foodborne pathogens.
Details
- Title
- A proof-of-concept study using a graphene oxide-bovine serum albumin nanocomposite-based electrochemical aptasensor platform for Salmonella typhimurium detection from chicken meat
- Authors/Creators
- Settasart Sonna - Chulalongkorn UniversityChatchawan Srisawat - Siriraj HospitalSakda Jampasa - Walailak UniversityOrawon Chailapakul - Chulalongkorn UniversityDavid J Hampson - School of Veterinary Medicine, Murdoch University, Perth, AustraliaNuvee Prapasarakul - Chulalongkorn University
- Publication Details
- The Veterinary quarterly, Vol.45(1), 2591482
- Publisher
- Informa UK limited, trading as Taylor & Francis Group
- Number of pages
- 17
- Identifiers
- 991005833798907891
- Copyright
- © 2025 the author(s).
- Murdoch Affiliation
- School of Veterinary Medicine
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Metrics
6 Record Views