Journal article
A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault
International Journal of Legal Medicine, Vol.131(1), pp.87-94
2017
Abstract
Sexual assault samples are some of the most common samples encountered in forensic analysis. These samples can require a significant time investment due to differential extraction processes. We report on the first record of successful direct amplification of semen for STR analysis. Neat seminal fluid, dilutions ranging from 1:5 to 1:160 and GEDNAP samples were successfully amplified using a direct method. A mild differential isolation technique to enrich spermatozoa was developed and successfully implemented to separate and directly amplify a mixture of semen and female epithelial cells. Aliquots of samples subjected to the differential isolation protocol were stained with Haemotoxylin and Eosin for sperm scoring. Samples stained after PCR showed a complete lack of intact spermatozoa demonstrating that the cells are lysed during the PCR process. This paper demonstrates the potential to incorporate direct PCR in cases of sexual assault to more rapidly obtain results and achieve a higher sensitivity.
Details
- Title
- A proof of principal study on the use of direct PCR of semen and spermatozoa and development of a differential isolation protocol for use in cases of alleged sexual assault
- Authors/Creators
- S.S. Tobe (Author/Creator)Y.C. Swaran (Author/Creator)L. Dennany (Author/Creator)U. Sibbing (Author/Creator)K. Schulze Johann (Author/Creator)L. Welch (Author/Creator)M. Vennemann (Author/Creator)
- Publication Details
- International Journal of Legal Medicine, Vol.131(1), pp.87-94
- Publisher
- Springer Berlin Heidelberg
- Identifiers
- 991005543797307891
- Copyright
- © 2016 Verlag Berlin Heidelberg
- Murdoch Affiliation
- Murdoch University
- Language
- English
- Resource Type
- Journal article
UN Sustainable Development Goals (SDGs)
This output has contributed to the advancement of the following goals:
Source: InCites
Metrics
103 Record Views
InCites Highlights
These are selected metrics from InCites Benchmarking & Analytics tool, related to this output
- Collaboration types
- Domestic collaboration
- International collaboration
- Citation topics
- 1 Clinical & Life Sciences
- 1.189 Genome Studies
- 1.189.310 Population Genetics
- Web Of Science research areas
- Medicine, Legal
- ESI research areas
- Clinical Medicine